The lymph nodes draining the small intestine and colon are anatomically separate and immunologically distinct
The lymph nodes draining the small intestine and colon are anatomically separate and immunologically distinct
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DOI：
10.1038/mi.2015.77
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发表时间：
2016-03-01
影响因子：
8
通讯作者：
Milling, S.
中科院分区：
文献类型：
--
作者：
Houston, S. A.;Cerovic, V.;Milling, S.
小肠(SI)和结肠中的树突状细胞(DC)是直接肠道免疫反应的基础；它们迁移到肠系膜淋巴结(MLN)和原始T细胞。我们证明了肠淋巴引流的解剖隔离，特别是来自SI和结肠的DC迁移到MLN内的不同节点，这里称为SMLN和cMLN。因此，在SI和结肠中观察到的DC亚集的不同频率在SMLN和cMLN中的DC之间反映。与SI吸收食物的功能一致，FED抗原存在于SMLN中，而不存在于cMLN中。此外，与cMLN相比，SMLN中T细胞上CCR9和α4β7的表达水平增加。来自cMLN和结肠的DC不能将维生素A代谢成维甲酸(RA)；因此，DC可能是SMLN和cMLN中观察到的组织归巢标志物表达差异的原因之一。总之，SMLN和cMLN以及迁移到这些LNS的DC在解剖学和免疫学上是分开的。这种隔离使SI和结肠中的免疫反应可以独立控制。
Dendritic cells (DCs) in the small intestine (SI) and colon are fundamental to direct intestinal immune responses; they migrate to the mesenteric lymph nodes (MLNs) and prime Tcells. We demonstrate anatomical segregation of lymphatic drainage from the intestine, specifically that DCs from the SI and colon migrate to different nodes within the MLN, here called the sMLN and cMLN. As a consequence, different frequencies of DC subsets observed in the SI and colon are reflected among the DCs in the sMLN and cMLN. Consistent with the SI's function in absorbing food, fed antigen is presented in the sMLN, but not in the cMLN. Furthermore, the levels of expression of CCR9 and alpha 4 beta 7 are increased on Tcells in the sMLN compared with the cMLN. DCs from the cMLN and colon are unable to metabolize vitamin A to retinoic acid (RA); thus, DCs may contribute to the differential expression of tissue homing markers observed in the sMLN and cMLN. In summary, the sMLN and cMLN, and the DCs that migrate to these LNs are anatomically and immunologically separate. This segregation allows immune responses in the SI and colon to be controlled independently.