DAY-NIGHT AND CIRCADIAN-RHYTHM CONTROL OF CON GENE-EXPRESSION IN NEUROSPORA
DAY-NIGHT AND CIRCADIAN-RHYTHM CONTROL OF CON GENE-EXPRESSION IN NEUROSPORA
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DOI：
10.1073/pnas.90.17.8249
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发表时间：
1993-09-01
影响因子：
11.1
通讯作者：
YANOFSKY, C
中科院分区：
文献类型：
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作者：
LAUTER, FR;YANOFSKY, C
在丝状真菌粗糙脉孢菌（Neurosporacrassa）中，孢子形成过程中的几个事件都受到孢子萌发的影响。两个基因，CON-6和CON-10，这是以前被证明是转录激活的分生孢子过程中，暴露于光，被发现是不表达的菌丝体保持在恒定的黑暗或恒定的光。而当菌丝体从黑暗中剥离到光照下时，这两个基因的转录本都大量出现。在进一步的照明两个转录消失，即，他们的继续生产受到了轻微的抑制。当黑暗生长的菌丝体暴露于光脉冲，并在黑暗中重新孵育，表达的con-6和con-10表现出20小时的昼夜节律周期性。这两个基因是光诱导的整个阶段的昼夜节律。在突变株bd和bd; frq 9中，con-6和con-10是光诱导的，但通常不是光抑制的。突变基因，如acon-2，acon-3，和fl，阻断con-6和/或con-10的发育表达，并没有阻止他们的光诱导。
In the filamentous fungus Neurospora crassa, several events in the process of conidiation are influenced by fight. Two genes, con-6 and con-10, which were previously shown to be transcriptionally activated during conidiation and by exposure to light, were found to be unexpressed in mycelium maintained in constant darkness or in constant light. However, when mycelium was shffted from darkness to light, transcripts of both genes appeared and were abundant. Upon further illumination both transcripts disappeared-i.e., their continued production was light repressed. When dark-grown mycelium was exposed to a light pulse and reincubated in the dark, expression of con-6 and con-10 exhibited a 20-hr circadian periodkity. Both genes were photoinducible throughout the stages of the circadian cyde. In the mutant strains bd and bd;frq9, con-6 and con-10 were light inducible but were not normally light repressible. Mutant genes such as acon-2, acon-3, and fl that block developmental expression of con-6 and/or con-10 did not prevent their photoinduction.