THE USE OF HYDROPHOBIC SYNTHETIC GLYCOSIDES AS ACCEPTORS IN GLYCOSYLTRANSFERASE ASSAYS
THE USE OF HYDROPHOBIC SYNTHETIC GLYCOSIDES AS ACCEPTORS IN GLYCOSYLTRANSFERASE ASSAYS
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DOI：
10.1007/bf01048331
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发表时间：
1988-01-01
影响因子：
3
通讯作者：
HINDSGAUL, O
中科院分区：
文献类型：
--
作者：
PALCIC, MM;HEERZE, LD;HINDSGAUL, O
描述了一种测定糖基转移酶活性的通用方法，该方法利用连接到疏水糖苷配基的合成糖苷受体。酶与受体和放射性标记的糖核苷酸孵育形成的产物可通过吸附到反相C-18柱上快速（1分钟）与干扰放射性分离。在水洗涤后，通过用甲醇洗脱容易地分离产物。该方法可用于β（1-4）半乳糖基转移酶、α（1-2）岩藻糖基转移酶和N-乙酰氨基葡萄糖基转移酶I和V的测定。
A general method is described for the assay of glycosyltransferase activity, which makes use of synthetic glycoside acceptors attached to hydrophobic aglycones. The products formed by incubation of an enzyme with acceptor and radiolabelled sugarnucleotide can then be rapidly (one minute) separated from interfering radioactivity by adsorption on to reverse-phase C-18 cartridges. After aqueous washing, products are easily isolated by elution with methanol. The utility of the method for the assay of β(1–4)galactosyltransferase, α(1–2)fucosyltransferase andN-acetylglucosaminyltransferase I and V is demonstrated.