Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing.
Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing.
复制标题
利用微阵列分析和 SBS 测序鉴定和表征对白粉病感染和热应激敏感的小麦长非蛋白编码 RNA
DOI：
10.1186/1471-2229-11-61
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发表时间：
2011-04-07
影响因子：
5.3
通讯作者：
Sun Q
中科院分区：
文献类型：
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作者：
Xin M;Wang Y;Yao Y;Song N;Hu Z;Qin D;Xie C;Peng H;Ni Z;Sun Q
背景白粉病侵染、高温等生物和非生物胁迫是小麦产量和品质的重要限制因素。越来越多的证据表明，长的非蛋白编码RNA(NpcRNAs)在植物的发育和逆境反应中起着重要的作用。然而，对长npcRNAs的鉴定仅限于少数几种植物，如拟南芥、水稻和玉米，关于小麦长npcRNAs及其对非生物和生物胁迫响应的系统鉴定尚未见报道。结果在本研究中，通过计算分析和实验方法，我们获得了125个推测的小麦逆境响应长npcRNAs，它们在植物中不保守。其中一些是小RNA的前体，如microRNAs和siRNAs，两个长的npcRNAs被鉴定为信号识别粒子(SRP)7S RNA变体，三个被鉴定为U3 snoRNAs。我们发现小麦长npcRNAs具有组织依赖性的表达模式，并且对白粉病侵染和热胁迫有反应。结论不同的小麦长npcRNAs对白粉病侵染和热胁迫都有响应，并且在小麦对生物和非生物胁迫的反应中都有功能，这为进一步了解它们的功能和调控机制提供了一个起点。
BackgroundBiotic and abiotic stresses, such as powdery mildew infection and high temperature, are important limiting factors for yield and grain quality in wheat production. Emerging evidences suggest that long non-protein coding RNAs (npcRNAs) are developmentally regulated and play roles in development and stress responses of plants. However, identification of long npcRNAs is limited to a few plant species, such as Arabidopsis, rice and maize, no systematic identification of long npcRNAs and their responses to abiotic and biotic stresses is reported in wheat.ResultsIn this study, by using computational analysis and experimental approach we identified 125 putative wheat stress responsive long npcRNAs, which are not conserved among plant species. Among them, some were precursors of small RNAs such as microRNAs and siRNAs, two long npcRNAs were identified as signal recognition particle (SRP) 7S RNA variants, and three were characterized as U3 snoRNAs. We found that wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress.ConclusionOur results indicated that diverse sets of wheat long npcRNAs were responsive to powdery mildew infection and heat stress, and could function in wheat responses to both biotic and abiotic stresses, which provided a starting point to understand their functions and regulatory mechanisms in the future.
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影响因子：
3.7
作者：
Li L;Wang X;Sasidharan R;Stolc V;Deng W;He H;Korbel J;Chen X;Tongprasit W;Ronald P;Chen R;Gerstein M;Deng XW
通讯作者：
Deng XW
影响因子：
64.8
作者：
通讯作者：
--
使用组合微阵列分析秀丽隐杆线虫非编码 RNA 表达
影响因子：
14.9
作者：
He H;Cai L;Skogerbø G;Deng W;Liu T;Zhu X;Wang Y;Jia D;Zhang Z;Tao Y;Zeng H;Aftab MN;Cui Y;Liu G;Chen R
通讯作者：
Chen R
影响因子：
7.4
作者：
MacIntosh, GC;Wilkerson, C;Green, PJ
通讯作者：
Green, PJ
影响因子：
16
作者：
Lawrence, RJ;Earley, K;Pikaard, CS
通讯作者：
Pikaard, CS