Cloning and sequence analysis of the small subunit ribosomal RNA gene from Nematodirus battus.
Cloning and sequence analysis of the small subunit ribosomal RNA gene from Nematodirus battus.
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蝙蝠线虫小亚基核糖体 RNA 基因的克隆和序列分析。
DOI：
10.2307/3283773
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
F. Stringfellow
中科院分区：
文献类型：
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作者：
D. Zarlenga;J. Lichtenfels;F. Stringfellow
利用聚合酶链反应引物，利用扭曲血蜱（Haemonchus contortus）小亚基核糖体DNA （SSU rDNA）的5′和3′端互补，从扩增的基因组DNA中克隆出巴氏线虫（Nematodirus battus）小亚基核糖体RNA基因（SSU rRNA）。利用内部生成的引物对克隆的1758个碱基对片段进行了双向测序，并与Haemonchus属的碱基对序列进行了比对。结果表明，两属之间的序列差异为1.9%，其中巴氏线虫与秀丽隐杆线虫的序列差异为14.2%。本文提供的数据与先前发表的比较Haemonchus属rDNA序列的工作相结合，表明这组基因在这些亚家族之间的差异有限，并进一步表明该分子可能不适合单独用于评估毛线虫超家族的系统发育相关性。
The small subunit ribosomal RNA gene (SSU rRNA) from the nematode parasite, Nematodirus battus, was cloned from amplified genomic DNA using polymerase chain reaction primers complementary to the 5' and 3' ends of Haemonchus contortus small subunit ribosomal DNA (SSU rDNA). The 1,758 base pair cloned fragment was sequenced in both directions using internally generated primers and aligned with those from the genus Haemonchus. Results indicated a 1.9% sequence difference between the genera with a 14.2% difference between N. battus and Caenorhabditis elegans. Data presented here in conjunction with previously published work comparing rDNA sequences within the genus Haemonchus demonstrate limited divergence in this group of genes between these subfamilies and suggest further that this molecule alone may be inappropriate for assessing phylogenetic relatedness within the superfamily Trichostrongyloidea.