Global Analysis of the Impact of Deleting Trigger Factor on the Transcriptome Profile of Escherichia coli
Global Analysis of the Impact of Deleting Trigger Factor on the Transcriptome Profile of Escherichia coli
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删除触发因子对大肠杆菌转录组图谱影响的整体分析
DOI：
10.1002/jcb.25620
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发表时间：
2017-01-01
影响因子：
4
通讯作者：
Liu, Chuanpeng
中科院分区：
文献类型：
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作者：
Fan, Dongjie;Liu, Lushan;Liu, Chuanpeng
触发因子(Trigger Function，TF)是原核生物伴侣蛋白网络的重要组成部分，参与了新生多肽折叠、蛋白质转运、核糖体组装等多种基本细胞过程。为了更好地了解Tf的生理作用，我们测定了各种Tf缺失突变株的整体转录组图谱。我们发现，编码Tf的tig基因的缺失导致转录组的急剧变化，不仅影响到伴侣网络成员的基因表达，还改变了相当多与代谢和其他细胞过程相关的RNA的水平。进一步的研究表明，Tf结构域缺失的突变体仅能部分恢复这种改变，表明结构完整性对Tf的生物学功能是至关重要的。最后，通过结合转录组和表型结果，提出了Tf缺失对转录组图谱影响的生理机制。J.细胞。生物化学。2017年118：141-153。©2016 Wiley期刊，Inc.
Trigger factor (TF) is a key component of prokaryotic chaperone network, which is involved various basic cellular processes such as nascent peptide folding, protein trafficking, ribosome assembly. To better understanding the physiological roles of TF, global transcriptome profiles of a variety of TF deletion mutant strains of Escherichia coli were determined. We found that deletion of the tig gene, encoding TF, led to a dramatic alteration of transcriptome profile, not only affecting the gene expression of members of the chaperone network, but also changing the levels of quite a few RNAs related to metabolism and other cellular processes. Further studies showed that this alteration was only partially recovered by knockin of TF domain‐deletion mutants into the endogenous tig locus, indicating that structural integrity is crucial for the biological function of TF. Finally, by combining the transcriptome and phenotype results, a physiological mechanism underlying the impact of TF deletion on the transcriptome profile was proposed. J. Cell. Biochem. 118: 141–153, 2017. © 2016 Wiley Periodicals, Inc.