HIPPOCAMPAL 8-[H-3]HYDROXY-2-(DI-N-PROPYLAMINO)TETRALIN BINDING-SITE DENSITIES, SEROTONIN RECEPTOR (5-HT(1A)) MESSENGER-RIBONUCLEIC-ACID ABUNDANCE, AND SEROTONIN LEVELS PARALLEL THE ACTIVITY OF THE HYPOTHALAMOPITUITARY ADRENAL AXIS IN RAT
HIPPOCAMPAL 8-[H-3]HYDROXY-2-(DI-N-PROPYLAMINO)TETRALIN BINDING-SITE DENSITIES, SEROTONIN RECEPTOR (5-HT(1A)) MESSENGER-RIBONUCLEIC-ACID ABUNDANCE, AND SEROTONIN LEVELS PARALLEL THE ACTIVITY OF THE HYPOTHALAMOPITUITARY ADRENAL AXIS IN RAT
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DOI：
10.1111/j.1471-4159.1992.tb08348.x
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发表时间：
1992-09-01
影响因子：
4.7
通讯作者：
STERNBERG, EM
中科院分区：
文献类型：
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作者：
BURNET, PWJ;MEFFORD, IN;STERNBERG, EM
我们先前已经证明，与相对耐受的Fischer F344/N大鼠相比，Lewis大鼠对炎症性疾病的易感性与下丘脑-垂体-肾上腺轴对炎症和其他应激介质的低反应有关。由于5-羟色胺(5-羟色胺，5-羟色胺)和5-羟色胺(5-HT1a)受体是这一轴的重要刺激物，我们研究了Lewis、杂交的Harlan Spraogue Dawley和Fischer F344/N大鼠不同脑区的8-[H-3]-hydroxy-2，3-(di-n-propylamino)tetralin结合部位、5-HT1mRNA、5-羟色胺和5-羟基吲哚乙酸的水平。与Harlan SpragueDawley和Fischer F344/N大鼠相比，Lewis大鼠海马区和额叶皮质8-[H-3]hydroxy-2，3-(di-ni-propylamino)tetralin结合位点和5-HT1AmRNA的表达显著减少。肾上腺切除后，3株小鼠海马区8-[H-3]羟基-2，3-(二正丙氨基)四氢萘结合部位数和5-HT1a基因表达均增加。Fischer F344/N大鼠海马区5-羟色胺的水平显著高于Lewis和Harlan Spraogue Dawley大鼠在相同区域检测到的水平。Harlan Spraogue Dawley大鼠下丘脑5-羟色胺和5-羟基吲哚乙酸水平高于其他两个品系的相同区域。肾上腺切除增加了所有三个品系的下丘脑中5-羟基吲哚乙酸的水平。我们的结论是，大鼠海马5-HT1a受体密度和5-羟色胺水平与下丘脑-肾上腺轴的活性和反应性平行。
We have previously demonstrated that susceptibility of the Lewis rat to inflammatory disease, compared with the relatively resistant Fischer F344/N rat, is related to a hyporesponsive hypothalamopituitary-adrenal axis to inflammatory and other stress mediators. Because serotonin (5-HT) and the 5-HT1A receptor are important stimulators of this axis, we have investigated the levels of 8-[H-3]-hydroxy-2,3-(di-n-propylamino)tetralin binding sites, 5-HT1A mRNA, 5-HT, and 5-hydroxyindoleacetic acid in various brain regions of Lewis, outbred Harlan Sprague Dawley, and Fischer F344/N rats. Lewis rats expressed significantly fewer hippocampal and frontal cortical 8-[H-3]hydroxy-2,3-(di-ni-propylamino)tetralin binding sites and less 5-HT1A mRNA than Harlan Sprague Dawley and Fischer F344/N rats. Adrenalectomy increased the number of 8-[H-3]hydroxy-2,3-(di-n-propylamino)tetralin binding sites and 5-HT1A mRNA expression in the hippocampus of all three strains. Levels of hippocampal 5-HT in Fischer F344/N rats were significantly greater than levels detected in the same regions from Lewis and Harlan Sprague Dawley rats. Hypothalamic 5-HT and 5-hydroxyindoleacetic acid levels in Harlan Sprague Dawley rats were higher than the same area from the other two strains. Adrenalectomy increased the levels of 5-hydroxyindoleacetic acid in the hypothalamus of all three strains. We conclude that hippocampal 5-HT1A receptor densities and 5-HT levels in the rat parallel the activity and responsiveness of the hypothalamopituitary-adrenal axis.