Immunoelectron microscopic localization of fibrin-related antigen in human glomerular diseases.
Immunoelectron microscopic localization of fibrin-related antigen in human glomerular diseases.
复制标题
人类肾小球疾病中纤维蛋白相关抗原的免疫电子显微镜定位。
作者：
T. Ono;K. Kanatsu;T. Doi;K. Sekita;H. Nagai;E. Muso;H. Yoshida;T. Tamura;C. Kawai
应用抗人γ-链、α-链、μ-链和纤维蛋白原的抗血清，对9例特发性膜性肾病（MN）、8例微小病变型肾病综合征（MNS）和10例伊加肾病（IgA-N）患者的肾小球纤维蛋白相关抗原（FRA）分布进行了免疫电镜观察。在内皮、内皮下和/或电子致密沉积物（EDD）中观察到FRA的电子致密反应产物。在三种肾小球疾病中，MN的内皮细胞中FRA的电子致密反应产物的量最高。这表明在MN中在内皮上发生凝血。FRA的反应产物在IgA-N的系膜EDD中呈强染色，而在MN的上皮下EDD中呈弱染色。这表明FRA几乎不穿透到MN的上皮下EDD。
The distribution of fibrin-related antigen (FRA) in glomeruli was examined by immunoelectron microscopy in 9 patients with idiopathic membranous nephropathy (MN), 8 patients with minimal-change nephrotic syndrome, and 10 patients with IgA nephropathy (IgA-N), using antisera against human gamma--chain, alpha-chain, mu-chain, and fibrinogen. Electron-dense reaction products of FRA were observed in the endothelium, subendothelium, and/or in electron-dense deposits (EDD). Among the three glomerular diseases, the amount of electron-dense reaction products of FRA in the endothelium was highest in MN. This suggests that coagulation occurs on the endothelium in MN. Although the mesangial EDD of IgA-N were intensely stained with reaction products of FRA, the staining was weak in the subepithelial EDD of MN. This suggests that FRA hardly penetrates into the subepithelial EDD in MN.