3D printing and biocompatibility study of a new biodegradable occluder for cardiac defect
3D printing and biocompatibility study of a new biodegradable occluder for cardiac defect
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新型可生物降解心脏缺陷封堵器的3D打印及生物相容性研究
DOI：
10.1016/j.jjcc.2019.02.002
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发表时间：
2019-08-01
影响因子：
2.5
通讯作者：
Cao, Qian
中科院分区：
文献类型：
--
作者：
Sun, Yiming;Zhang, Xingjian;Cao, Qian
目的：目的：采用三维打印技术制备可生物降解的心脏缺损封堵器，并评价其在动物模型上的生物安全性。通过细胞毒性试验、溶血试验、动态凝血试验和血小板粘附试验评价材料的细胞学和血液学生物相容性。结果：封堵器样品打印效果良好。细胞毒性试验显示PLTG对细胞无明显毒性作用。溶血试验显示PLTG的溶血率小于5%，表明对红细胞仅有轻微影响。动态凝血试验显示内源性凝血因子活化不良。如血小板粘附试验所示，与PLLA相比，PLTG导致较低的血小板活化。结论：利用3D打印技术可以制备出一种生物相容性良好的新型PLTG基生物可降解心脏封堵器。(C)2019年日本心脏病学院。由爱思唯尔有限公司出版。保留所有权利。
Objective: To fabricate a biodegradable occluder for heart defect using the three-dimensional (3D) printing technique and evaluate its biosafety in an animal model.Methods: Occluder samples were made by 3D printing technique using the self-developed lactide-sanya methyl carbonate-glycolide (PLLA-TMC-GA) co-polymer or PLTG as the bio-material. The biocompatibility (cytological and hematological) of the materials was evaluated by cytotoxicity experiments, hemolysis test, dynamic blood clotting test, and platelet adhesion test. Finally, the histocompatibility of the occluder was evaluated by implantation in a rabbit model.Results: Occluder samples were printed satisfactorily. Cytotoxicity assay showed no significant toxicity of PLTG in the cells. Hemolysis test showed less than 5% hemolysis rate of PLTG indicating only a mild effect on the red blood cells. The dynamic coagulation test showed poor activation of endogenous clotting factors. PLTG resulted in lower platelet activation compared to PLLA, as indicated by the platelet adhesion test. Finally, no obvious tissue damage or necrosis was seen in the in vivo implantation experiment.Conclusion: A new PLTG-based biodegradable occluder for heart defects with good biocompatibility can be manufactured by the 3D printing technique. (C) 2019 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.