Quantification of magnetic susceptibility in several strains of Bacillus spores:: Implications for separation and detection
Quantification of magnetic susceptibility in several strains of Bacillus spores:: Implications for separation and detection
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DOI：
10.1002/bit.21400
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发表时间：
2007-09-01
影响因子：
3.8
通讯作者：
Chalmers, Jeffrey J.
中科院分区：
文献类型：
--
作者：
Melnik, Kristie;Sun, Jianxin;Chalmers, Jeffrey J.
三种芽孢杆菌菌株：测试了萎缩芽孢杆菌（正式的球形芽孢杆菌）、苏云金芽孢杆菌和蜡状芽孢杆菌的固有磁化率。所有三种菌株孢子化时表现出显着的磁化率使用称为细胞跟踪测速仪的仪器。能量色散光谱也证实了细菌孢子形式中存在顺磁性元素Fe和Mn。证明了这种磁化率足以在磁沉积系统中将这些孢子分离并存款在载玻片上。这些结果表明，分离孢子与固有的磁化率直接从水或空气样品的潜力。
Three strains of Bacillus: Bacillus atrophaeus (formally Bacillus globigii), Bacillus thuringiensis, and Bacillus cereus were tested for their intrinsic magnetic susceptibility. All three strains when sporulated demonstrated significant magnetic susceptibility using an instrument referred to as Cell Tracking Velocimetry. Energy dispersive spectroscopy also confirmed the presence of paramagnetic elements, Fe and Mn, in the spore form of the bacteria. It was demonstrated that this magnetic susceptibility is sufficient to separate and deposit these spores on glass slides in a magnetic deposition system. These results indicate the potential to separate spores with intrinsic magnetic susceptibility directly out of water or air samples.