Growth inhibition of Candida albicans hyphae by CD8+ lymphocytes.
Growth inhibition of Candida albicans hyphae by CD8+ lymphocytes.
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DOI：
10.4049/jimmunol.154.10.5273
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发表时间：
1995-05
影响因子：
4.4
通讯作者：
D. Beno;A. G. Stöver;H. Mathews
中科院分区：
文献类型：
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作者：
D. Beno;A. G. Stöver;H. Mathews
我们之前已经证明il -2激活的脾细胞可以在体外抑制白色念珠菌菌丝的生长。在这里，我们证明了CD8+的塑性非粘附淋巴细胞介导抗真菌活性。富集CD8+细胞可显著增强il -2活化淋巴细胞群对白色念珠菌的抗真菌活性和nk抗性细胞系淋巴细胞的细胞毒活性。CD8+细胞的消耗降低了nk抗性细胞系淋巴细胞群的抗真菌活性和细胞毒活性。NK1.1+细胞的富集显著降低了淋巴细胞群对白色念珠菌的抗真菌活性，提高了nk敏感细胞系淋巴细胞的细胞毒活性。NK1.1+细胞的缺失增加了nk抗性细胞系淋巴细胞群的抗真菌活性和细胞毒活性。培养中抗真菌淋巴细胞的生成需要IL-2，而不需要ifn - γ代替。这些数据表明，IL-2激活的CD8+ T淋巴细胞对白色念珠菌菌丝形式发挥最大的抗真菌作用，而IL-2或ifn - γ激活的NK细胞对菌丝的作用很小或没有作用。
We have shown previously that IL-2-activated splenocytes can inhibit the growth of Candida albicans hyphae in vitro. Herein we demonstrate that plastic nonadherent lymphocytes that are CD8+ mediate the antifungal activity. Enrichment for CD8+ cells markedly enhanced the antifungal activity of the IL-2-activated lymphocyte population for C. albicans and the cytotoxic activity of the lymphocytes for an NK-resistant cell line. Depletion of CD8+ cells reduced the lymphocyte population's antifungal activity and cytotoxic activity for the NK-resistant cell line. Enrichment for NK1.1+ cells markedly reduced the antifungal activity of the lymphocyte population for C. albicans and increased the cytotoxic activity of the lymphocytes for an NK-sensitive cell line. Depletion of NK1.1+ cells increased the lymphocyte population's antifungal activity and cytotoxic activity for the NK-resistant cell line. Generation of the antifungal lymphocytes in culture required IL-2 and was not replaced with IFN-gamma. These data show that IL-2-activated CD8+ T lymphocytes exert the greatest amount of antifungal effect against the hyphal form of C. albicans, whereas IL-2- or IFN-gamma-activated NK cells have little or no effect against the hyphae.