Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design
Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design
复制标题
DOI：
10.1016/j.biortech.2004.11.005
复制
发表时间：
2005-08-01
影响因子：
11.4
通讯作者：
Gunasekaran, P
中科院分区：
文献类型：
--
作者：
Senthilkumar, SR;Ashokkumar, B;Gunasekaran, P
采用响应面法和中心复合旋转设计法对费氏曲霉以麦皮为底物，在pH 9.0条件下固态发酵生产无碱稳定纤维素酶的发酵条件进行了优化。这项研究涉及的四个变量是亚硝酸钠、磷酸二氢钾、硫酸镁和酵母膏。对结果的统计分析表明，在研究的范围内，只有亚硝酸钠对木聚糖酶的产生有显著影响。在含有NaNO_2-7.0、K_2HPO_4-1.0、MgSO_4-0.5和酵母膏-5.0的优化培养基中，发酵72 h后，碱性木聚糖酶的产量(1024U/g麸皮)较初始水平(540U/g)提高了1.9倍，而二次模型预测值为931U/g。优化后的发酵条件下，木聚糖酶活性显著降低，从而保持了木聚糖酶的活力，证明了应用统计实验设计的另一个优点。(C)2005年由爱思唯尔有限公司出版。
Response surface methodology and central composite rotary design (CCRD) was employed to optimize a fermentation medium for the production of alkali-stable cellulase-free xylanase by Aspergillus fischeri in solid-state fermentation at pH 9.0 with wheat bran as substrate. The four variables involved in this study were sodium nitrite, potassium dihydrogen phosphate, magnesium sulphate and yeast extract. The statistical analysis of the results showed that, in the range studied, only sodium nitrite had a significant effect on xylanase production. The optimized medium containing (in g/l) NaNO2-7.0, K2HPO4-1.0, MgSO4-0.5 and yeast extract-5.0 resulted in 1.9-fold increased level of alkali-stable xylanase (1024 U/g wheat bran) production compared to initial level (540 U/g) after 72 h of fermentation, whereas its value predicted by the quadratic model was 931 U/g. The level of protease activity was considerably decreased in optimized medium, thus helping to preserve the xylanase activity and demonstrating another advantage of applying statistical experimental design. (c) 2005 Published by Elsevier Ltd.