In vitro fertilization and cleavage of human ovarian eggs
In vitro fertilization and cleavage of human ovarian eggs
复制标题
DOI：
10.1126/science.100.2588.105
复制
发表时间：
1944-07-01
期刊：
影响因子：
56.9
通讯作者：
Menkin, MF
中科院分区：
文献类型：
--
作者：
Rock, J;Menkin, MF
利用手术材料，在体外实现了3例不同病例中的受精和卵裂，这些病例是在预期排卵前从卵巢组织中获得的。鸡蛋在洛克的溶液中洗净，在同一个病人的血清中孵育，在室温下暴露。将洗涤过的精子悬浮液加入到洛克溶液中，并在[image]35放大倍数下进行检查。1小时后，将每个卵子转移到新鲜血清中并重新检查。受精后，2枚卵持续40 1/2和45小时，在2-细胞期，两个卵在3-细胞期46小时可见。在暴露于精子之后。
Using surgical material, fertilization and cleavage of human eggs in 3 different cases obtained from ovarian tissue just prior to expected ovulation were achieved in vitro. The eggs were washed in Locke''s soln., incubated in the serum of the same patient, exposed at room temp. to a washed sperm suspension in Locke''s soln., and examined at [image]35 magnification. After 1 hr. each ovum was transferred to fresh serum and re-examined. After fertilization, 2 eggs lasted 401/2 and 45 hrs., respectively, in the 2-cell stage, and both eggs were seen in the 3-cell stage 46 hrs. after exposure to sperm.