Cytologic, histologic, and immunohistochemical features of maxillofacial alveolar rhabdomyosarcoma in a juvenile dog
Cytologic, histologic, and immunohistochemical features of maxillofacial alveolar rhabdomyosarcoma in a juvenile dog
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DOI：
10.1111/j.1939-165x.2009.00175.x
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发表时间：
2010-03-01
影响因子：
1.2
通讯作者：
Masegi, Toshiaki
中科院分区：
文献类型：
--
作者：
Murakami, Mami;Sakai, Hiroki;Masegi, Toshiaki
一只15个月大的阉割公犬，有间歇性鼻出血和打喷嚏的病史，因颌面部肿块接受检查。花菜状牙龈肿块的活检样本的印象涂片含有大量圆形细胞，直径5-25 μ m，其中含有适量的透明至淡蓝色细胞质，类似淋巴样细胞。经常观察到有丝分裂象。肿块诊断为恶性圆细胞瘤。组织学检查肿瘤由弥漫性排列的非典型小圆细胞和少量纤维血管间质组成。免疫组化结果显示，细胞CD3、CD18、CD20、CD79 α、细胞角蛋白、黑色素A、嗜铬粒蛋白A、-平滑肌肌动蛋白和肌红蛋白均呈阴性，而波形蛋白和desmin呈阳性。细胞中肌原素和MyoD1的核染色也呈强阳性。根据形态学和免疫组织化学结果诊断为实型肺泡横纹肌肉瘤。在幼年犬的肿瘤鉴别诊断中应考虑肺泡横纹肌肉瘤，特别是当细胞学结果显示圆形未分化细胞时。
A 15-month-old castrated male dog with a history of intermittent epistaxis and sneezing was admitted for the examination of a maxillofacial mass. An impression smear of a biopsy sample from the cauliflower-shaped gingival mass contained numerous round cells, 5-25 mu m in diameter, which contained a moderate amount of clear to pale blue cytoplasm and resembled lymphoid cells. Mitotic figures were frequently observed. The mass was diagnosed as malignant round cell neoplasia. On histologic examination the tumor was composed of diffusely arranged, small, atypical round cells with a small amount of fibrovascular stroma. Immunohistochemically, the cells were negative for CD3, CD18, CD20, CD79 alpha, cytokeratin, melan-A, chromogranin A, alpha-smooth muscle actin, and myoglobin but positive for vimentin and desmin. The cells also had strong positive nuclear staining for myogenin and MyoD1. A diagnosis of solid-pattern alveolar rhabdomyosarcoma was made on the basis of morphologic and immunohistochemical results. Alveolar rhabdomyosarcoma should be considered in the differential diagnosis of tumors in juvenile dogs, especially when cytologic findings reveal round, undifferentiated cells.