Construction of telocentric chromosomes in Saccharomyces cerevisiae.
Construction of telocentric chromosomes in Saccharomyces cerevisiae.
复制标题
酿酒酵母端粒染色体的构建。
DOI：
10.1073/pnas.82.7.2106
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发表时间：
1985
影响因子：
11.1
通讯作者：
Tye,BK
中科院分区：
文献类型：
--
作者：
Surosky,RT;Tye,BK
我们描述了一种简单的方法，用于在酵母中构建大的染色体缺失。用DNA片段转化二倍体酵母细胞，所述DNA片段通过同源重组替换染色体的大区域。使用这种方法，我们已经构建了一个端着丝粒染色体III，其中约等于100个碱基（kb）的DNA已被删除的左臂的染色体，使着丝粒是12 kb的左端粒。这条端部着丝粒染色体是有丝分裂稳定的。在二倍体品系中，其丢失率为每次细胞分裂2.5-7.4 × 10 ~（-4），而正常染色体III的丢失率为每次细胞分裂0.36-1.8 × 10 ~（-4）。在减数分裂过程中，它也以保真度分离2+：2-。在染色体中构建系统性缺失对于确定正确的染色体分离和复制的基本特征应该是有用的。
We describe a simple method for the construction of large chromosomal deletions in yeast. Diploid yeast cells were transformed with DNA fragments that replace large regions of the chromosomes by homologous recombination. Using this method, we have constructed a telocentric chromosome III in which approximately equal to 100 kilobases (kb) of DNA has been removed from the left arm of the chromosome, so that the centromere is 12 kb from the left telomere. This telocentric chromosome is mitotically stable. Its rate of loss in a diploid strain is 2.5-7.4 X 10(-4) per cell division compared to a rate of loss of 0.36-1.8 X 10(-4) per cell division for a normal chromosome III. It also segregates 2+:2- with fidelity during meiosis. The construction of systematic deletions in a chromosome should be useful in determining the essential features for proper chromosomal segregation and replication.