The replication of measles virus in the presence of tunicamycin.
The replication of measles virus in the presence of tunicamycin.
复制标题
衣霉素存在下麻疹病毒的复制。
DOI：
10.1016/0042-6822(81)90447-5
复制
发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Fields,BN
中科院分区：
文献类型：
--
作者：
Stallcup,KC;Fields,BN
麻疹的糖蛋白参与许多生物学活动，包括传染性和细胞融合。为了研究碳水化合物在病毒糖蛋白功能中的作用，使用衣霉素（TM）来抑制糖添加到糖蛋白中。在感染病毒后2小时，每毫升注射1微克该药，可使感染性子代病毒的产生减少103- 104。这种抑制发生在33或37°，并影响埃德蒙斯顿和乔伊野生型麻疹菌株。病毒粒子密度的物质释放被抑制85- 90%。在药物存在下，非糖基化病毒蛋白（L、P、NP、M）的合成似乎是正常的，而在H（大病毒糖蛋白）和融合蛋白的F1 a组分的合成中观察到变化。从TM处理的培养物释放的颗粒缺乏正常的H和F1蛋白。病毒体制剂含有一种多肽，称为p90，它比H在SDS-PAGE上迁移更慢，也发现在2-脱氧-d-葡萄糖存在下感染的培养物释放的颗粒制剂中。正常培养物在感染后期用[35 S]甲硫氨酸脉冲2小时，释放出已将标记掺入L、p90、P、NP和M多肽中的病毒粒子。
The glycoproteins of measles are involved in a number of biological activities, including infectivity and cell fusion. In order to study the role of carbohydrates in the function of viral glycoproteins, tunicamycin (TM) was used to inhibit the addition of sugars to glycoproteins. One microgram per milliliter of the drug, administered 2 hr postinfection to cell cultures infected with virus, caused a 103–104reduction in the production of infectious progeny virus. This inhibition occurred at either 33 or 37° and affected both the Edmonston and Joy wild-type measles strains. The release of material with the density of virions was inhibited 85–90%. Synthesis of the nonglycosylated viral proteins (L, P, NP, M) appeared to be normal in the presence of the drug, while changes were observed in the synthesis of H, the large viral glycoprotein, and F1a component of the fusion protein. Particles released from TM-treated cultures lacked the normal H and F1proteins. Virion preparations contained a polypeptide, termed p90, which migrated more slowly than H on SDS-PAGE and was also found in preparations of particles released from cultures infected in the presence of 2-deoxy-d-glucose. Normal cultures pulsed for 2 hr with [35S]methionine late in infection released virions that had incorporated the label into the L, p90, P, NP, and M polypeptides.