Kinetic Analysis of Photosterilization Rate of Escherichia coli Cells in Titanium Dioxide Suspension Associated with Redox Reactions of Iron Ions
Kinetic Analysis of Photosterilization Rate of Escherichia coli Cells in Titanium Dioxide Suspension Associated with Redox Reactions of Iron Ions
复制标题
二氧化钛悬浮液中大肠杆菌细胞光杀菌率与铁离子氧化还原反应的动力学分析
DOI：
10.1252/jcej.34.1381
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发表时间：
2001
影响因子：
0.8
通讯作者：
M. Taya
中科院分区：
文献类型：
--
作者：
Y. Koizumi;Jumpei Nishi;M. Taya
研究了以TiO 2粒子为光杀菌剂，以Fe ~（3+）为电子受体对大肠杆菌细胞的光杀菌作用。当Fe ~（3+）初始浓度为0.1，30和100 × 10 - 3 mol/m3时，细胞失活，而当Fe ~（3+）不存在时，细胞不失活。在平均光照强度为0-22 W/m2，TiO 2浓度为0 ~ 0.1kg/m3的条件下，测定了表观杀菌速率常数k '。当CTO = 0.0 1 kg/m3时，k '/Iobs的斜率为3.8 × 10 - 5 m2/J，k'/Iobs随TiO 2浓度的增加而增加，在CTO = 0.1kg/m3时，k '/Iobs的斜率为8.0 × 10 - 5 m2/J，k'/Iobs随TiO 2浓度的增加而增加，在CTO = 0.1kg/m3时达到饱和。13和22 W/m 2。这种k '/IObs值与CTO值之间的关系可以从动力学上用Fe、Fe离子的光反应和TiO 2颗粒在E.大肠杆菌细胞。
Photosterilization of Escherichia coli cells was investigated using TiO 2 particles in the presence of ferric ion as an electron acceptor. Deactivation of the cells was recognized in the reaction mixture containing ferric ion at the initial concentration of C Fe3,0 1, 30 and 100 x 10 -3 mol/m 3 and the cell deactivation did not occur in the absence of ferric ion. The apparent sterilization rate constant, k', was determined from the photoreaction tests conducted under the varied conditions of average light intensity (Ī obs = 0-22 W/m 2 ) and TiO 2 concentration (C TO = 0-0.1 kg/m 3 ). A proportional relation was obtained between the values of k' and Ī obs with the slope of k'/ Ī obs = 3.8 x 10 -5 m 2 /J at C TO = 0.01 kg/m 3 , while the value of k'/ I obs increased with an increase in TiO 2 concentration depicting a saturated profile up to C TO = 0.1 kg/m 3 at Ī obs = 8, 13 and 22 W/m 2 . This relation between the values of k'/ I obs and C TO could he kinetically explained by considering the photoreactions of ferric and ferrous ions and the adsorption of TiO 2 particles onto E. coli cells in the reaction mixture.