Tensile Loads on Tethered Actin Filaments Induce Accumulation of Cell Adhesion-Associated Proteins in Vitro.
Tensile Loads on Tethered Actin Filaments Induce Accumulation of Cell Adhesion-Associated Proteins in Vitro.
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拴系肌动蛋白丝上的拉伸载荷会在体外诱导细胞粘附相关蛋白的积累。
DOI：
10.1021/acs.langmuir.8b02076
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发表时间：
2019
期刊：
影响因子：
3.9
通讯作者：
and M. Sokabe. 2018
中科院分区：
文献类型：
--
作者：
Kiyoshima;D.;H. Tatsumi;H. Hirata;and M. Sokabe. 2018
分别作为细胞-基质和细胞-细胞相互作用的机械界面的粘着斑（FA）和粘着连接（AJs）经历源自周围组织的变形或由细胞中的肌动球蛋白机制产生的张力。这些机械输入导致FAs和AJs的扩大，而FAs和AJs的力依赖性发展的详细机制仍不清楚。FA和AJs都为肌动蛋白丝的束缚和肌动蛋白聚合提供了位点。在这里，我们开发了一个无细胞的系统，其中肌动蛋白丝被拴在玻璃表面，并表明，张力的应用程序中的细胞提取物中的拴丝诱导积累的几个FA和AJ蛋白，与进一步积累的肌动蛋白丝通过从头肌动蛋白聚合。张力的下降导致积累的蛋白质的量减少。这些结果表明，张力作用于拴系的肌动蛋白丝起着至关重要的作用，在积累的FA和AJ蛋白。
Focal adhesions (FAs) and adherens junctions (AJs), which serve as a mechanical interface of cell–matrix and cell–cell interactions, respectively, experience tensile force either originating from the deformation of the surrounding tissues or generated by the actomyosin machinery in the cell. These mechanical inputs cause enlargement of FAs and AJs, while the detailed mechanism for the force-dependent development of FAs and AJs remain unclear. Both FAs and AJs provide sites for tethering of actin filaments and actin polymerization. Here, we develop a cell-free system, in which actin filaments are tethered to glass surfaces, and show that application of tensile force to the tethered filaments in the cell extract induces accumulation of several FA and AJ proteins, associated with further accumulation of actin filaments via de novo actin polymerization. Decline in the tensile force results in a decrease in the amount of the accumulated proteins. These results suggest that the tensile force acting on the tethered actin filaments plays a crucial role in the accumulation of FA and AJ proteins.