SENSITIVE ANALYSIS OF THE MYCOTOXIN ZEARALENONE AND ITS METABOLITES IN BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
SENSITIVE ANALYSIS OF THE MYCOTOXIN ZEARALENONE AND ITS METABOLITES IN BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0378-4347(00)82675-4
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发表时间：
1989-09-29
期刊：
影响因子：
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通讯作者：
TRENHOLM, HL
中科院分区：
文献类型：
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作者：
PRELUSKY, DB;WARNER, RM;TRENHOLM, HL
本文介绍了一种用高效液相色谱-荧光检测法分析牛奶、血液、血浆、尿液和胆汁等小体积(0.5-2.0毫升)生物体液中玉米赤霉烯酮及其代谢物α-和β-玉米赤霉烯醇的方法。通过一系列pH控制的溶剂萃取法实现了从生物液中分离毒素。玉米赤霉烯酮和α-玉米赤霉烯醇的检出限为1 ng/ml，β-玉米赤霉烯醇的检出限约为5 ng/ml，两者的信噪比均为3。然而，在胆汁中，由于干扰物质的存在，检测灵敏度降低了约5倍。低浓度时，尿液(87-94%)和血浆(85-93%)的回收率最高，全血(78-88%)和牛奶(75-84%)的回收率稍低，胆汁(66-77%)的回收率最低。
A method is described for the analysis of zearalenone and its metabolites, .alpha.- and .beta.-zearalenol, in small volumes (0.5-2.0 ml) of biological fluids including milk, blood, plasma, urine and bile, using high-performance liquid chromatography with fluorescence detection. Isolation of the toxins from biological fluids was achieved using a series of pH-controlled solvent extractions. Detection limits for zearalenone and .alpha.-zearalenol were 1 ng/ml, and for .beta.-zearalenol ca. 5 ng/ml, both at a signal-to-noise ratio of 3. In bile, however, the detection was ca. five times less sensitive owing to interfering substances. Recoveries at low ng/ml concentrations were highest from urine (87-94%) and plasma (85-93%), slightly lower from whole blood (78-88%) and milk (75-84%), and lowest from bile (66-77%).