Antihyperglycemic Effects of Baicalin on Streptozotocin - Nicotinamide induced Diabetic Rats
Antihyperglycemic Effects of Baicalin on Streptozotocin - Nicotinamide induced Diabetic Rats
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DOI：
10.1002/ptr.3238
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发表时间：
2011-02-01
影响因子：
7.2
通讯作者：
Wang, Jiping
中科院分区：
文献类型：
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作者：
Li, Huan-Ting;Wu, Xiao-Dong;Wang, Jiping
本研究旨在探讨黄芩苷对大鼠血糖、胰岛素及细胞因子的影响。采用烟酰胺和链脲佐菌素腹腔注射诱导大鼠糖尿病。糖尿病大鼠每日ig黄芩苷或口服二甲双胍，连续8 d。采用常规方法测定血糖、胰岛素、肝糖原。用葡萄糖-6-磷酸脱氢酶偶联法测定肝己糖激酶的活性。采用酶联免疫吸附法测定血清白细胞介素-6 （IL-6）、肿瘤坏死因子- α (tnf - α)和脂联素水平。以50或100)mg/kg剂量给药的黄芩苷显著降低血糖水平，且呈剂量依赖性。黄芩苷未显著提高血清胰岛素水平。大剂量黄芩苷（100 mg/kg）可显著增加肝糖原含量，降低血清tnf - α。黄芩苷在25、50、10 mg/kg剂量下，肝己糖激酶活性显著升高。黄芩苷（50、10 mg/kg）和二甲双胍（10 mg/kg）可显著减轻STZ对胰腺的形态学损伤。降糖作用的可能机制包括增加肝糖原含量和糖酵解，降低血清tnf - α水平。版权所有John Wiley & Sons， Ltd。
The aim of the study was to investigate the effects of baicalin on blood glucose, insulin and cytokine levels. Rat diabetes was induced by intraperitoneal (i.p.) injection of nicotinamide and streptozotocin. Diabetic rats were dosed with i.p. baicalin or oral metformin daily for 8 days. Blood glucose, insulin and hepatic glycogen were determined using conventional methods. The activity of hepatic hexokinase was determined using a coupled assay with glucose-6-phosphate dehydrogenase. Serum levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) and adiponectin were measured by enzyme-linked immunosorbent assay. Administration of baicalin at 50 or 100) mg/kg significantly decreased plasma glucose levels in a dose dependent manner. The serum insulin level was not increased by baicalin treatment. Administration of baicalin at a high dose (100 mg/kg) resulted in a significant increase of liver glycogen content and a reduction of serum TNF-alpha. The activity of hepatic hexokinase was significantly increased after dosing baicalin at 25, 50 or 10 mg/kg. Administration of baicalin (50 or 10 mg/kg) or metformin (10 mg/kg) significantly alleviated the morphological injury to the pancreas caused by STZ. The possible mechanisms contributing to the hypoglycemic effect include increasing the hepatic glycogen content and glycolysis, and reducing the serum levels of TNF-alpha. Copyright (C) 2010 John Wiley & Sons, Ltd.