Connexin43 regulates joint location in zebrafish fins
Connexin43 regulates joint location in zebrafish fins
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DOI：
10.1016/j.ydbio.2008.12.027
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发表时间：
2009-03-15
影响因子：
2.7
通讯作者：
Iovine, M. Kathryn
中科院分区：
文献类型：
--
作者：
Sims, Kenneth, Jr.;Eble, Diane M.;Iovine, M. Kathryn
关节对于骨骼的形态和功能是必不可少的，但它们的发育仍然知之甚少。在斑马鱼鳍，关节之间的骨鳍条段提供基本上无限的机会，以评估关节形态。cx43突变导致短鳍（Sof（b123））突变体的短节表型，表明直接细胞间通讯可能调节关节位置。有趣的是，在另一个长鳍（ALF（DTY 86））突变体中增加的CX 43表达似乎引起该突变体典型的关节衰竭。事实上，alf（dty86）突变体fins中cx43的敲低挽救了关节形成。总之，这些数据揭示了鳍条间充质中Cx43表达水平与关节位置之间的相关性。Cx43也被观察到横向与发展中的关节相关的细胞。共聚焦显微镜显示，Cx43蛋白最初包围的ZNS 5阳性关节细胞的膜，但在后期阶段成为极化向底层Cx43阳性间充质细胞。一种可能性是，Cx43阳性间充质和覆盖的ZNS 5阳性细胞之间的通信调节关节的位置，并上调Cx43在关节形成细胞有助于关节形态发生。(C)2008年爱思唯尔公司All rights reserved.
Joints are essential for skeletal form and function, yet their development remains poorly understood. In zebrafish fins, joints form between the bony fin ray segments providing essentially unlimited opportunities to evaluate joint morphogenesis. Mutations in cx43 cause the short segment phenotype of short fin (Sof(b123)) mutants, suggesting that direct cell-cell communication may regulate joint location. interestingly, increased cx43 expression in the another long fin (alf(dty86)) mutant appears to cause joint failure typical of that mutant. Indeed, knockdown of cx43 in alf(dty86) mutant fins rescues joint formation. Together, these data reveal a correlation between the level of Cx43 expression in the fin ray mesenchyme and the location of joints. Cx43 was also observed laterally in cells associated with developing joints. Confocal microscopy revealed that the Cx43 protein initially surrounds the membranes of ZNS5-positive joint cells, but at later stages becomes polarized toward the underlying Cx43-positive mesenchymal cells. One possibility is that communication between the Cx43-positive mesenchyme and the overlying ZNS5-positive cells regulates joint location, and upregulation of Cx43 in joint-forming cells contributes to joint morphogenesis. (C) 2008 Elsevier Inc. All rights reserved.