A Novel Chinese Honey from Amorpha fruticosa L.: Nutritional Composition and Antioxidant Capacity In Vitro.
A Novel Chinese Honey from Amorpha fruticosa L.: Nutritional Composition and Antioxidant Capacity In Vitro.
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DOI：
10.3390/molecules25215211
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发表时间：
2020-11-09
期刊：
影响因子：
--
通讯作者：
Cao W
中科院分区：
文献类型：
--
作者：
Zhu M;Zhao H;Wang Q;Wu F;Cao W
假靛蓝（Amorpha fruticosa L.， A. fruticosa）是中国西北地区防风防沙的首选乡土树种，但其蜂蜜的营养和生物活性特性资料较少。在西北地区采集了12种紫穗槐（Amorpha fruticosa L.， AFH）蜂蜜，对其营养成分进行了测定。采用电感耦合等离子体质谱法（ICP-MS）和高效液相色谱-四极杆飞行时间质谱法（HPLC-QTOF-MS）分别测定了16种矿物元素和10种优势多酚的含量。此外，AFH具有较高的DPPH（1,1-二苯基-2-吡啶肼基）自由基清除能力（IC50 100.41±15.35 mg/mL）、铁还原抗氧化能力（2.04±0.29µmol FeSO4·7H2O/g）和铁离子螯合能力（82.56±16.01 mg Na2EDTA/kg），与总酚含量（270.07±27.15 mg GA/kg）和抗坏血酸含量（213.69±27.87 mg/kg）显著相关。细胞模型验证了AFH在氧化应激反应中对pBR322质粒DNA和小鼠淋巴细胞DNA损伤具有剂量依赖性的预防作用。综上所述，我们的研究结果为AFH作为一种潜在的抗氧化膳食在食品工业中的应用提供了证据。
False indigo (Amorpha fruticosa L., A. fruticosa) is the preferred tree indigenous for windbreak and sand control in Northwest China, while information on nutritional and bioactive characteristics of its honey is rare. Herein, 12 honey of Amorpha fruticosa L. (AFH) were sampled in Northwest China and the nutritional composition was determined. Sixteen mineral element and ten dominant polyphenols content were identified and quantified by ICP-MS (Inductively coupled plasma mass spectrometry) and HPLC-QTOF-MS (High performance liquid chromatography-Quadrupole time-of-flight mass spectrometry), respectively. Moreover, AFH demonstrated high levels of DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging activity (IC50 100.41 ± 15.35 mg/mL), ferric reducing antioxidant power (2.04 ± 0.29 µmol FeSO4·7H2O/g), and ferrous ion-chelating activity (82.56 ± 16.01 mg Na2EDTA/kg), which were significantly associated with total phenolic contents (270.07 ± 27.15 mg GA/kg) and ascorbic acid contents (213.69 ± 27.87 mg/kg). The cell model verified that AFH exhibited dose-dependent preventive effects on pBR322 plasmid DNA and mouse lymphocyte DNA damage in response to oxidative stress. Taken together, our findings provide evidence for the future application of AFH as a potential antioxidant dietary in food industry.
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影响因子：
4
作者：
Afroz, Rizwana;Tanvir, E. M.;Khalil, Md. Ibrahim
通讯作者：
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DOI：
10.1016/s1044-0305(01)00226-4
发表时间：
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影响因子：
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作者：
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