The kinetochore protein Moa1 enables cohesion-mediated monopolar attachment at meiosis I
The kinetochore protein Moa1 enables cohesion-mediated monopolar attachment at meiosis I
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DOI：
10.1016/j.cell.2005.09.013
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发表时间：
2005-12-02
期刊：
影响因子：
64.5
通讯作者：
Watanabe, Y
中科院分区：
文献类型：
--
作者：
Yokobayashi, S;Watanabe, Y
减数分裂类似于有丝分裂，但采用独特的“还原”核分裂，允许从二倍体细胞产生单倍体配子。关键的倍性减少步骤需要姐妹动粒附着在从同一纺锤体极发出的微管上，实现“单极附着”，这确保了母本和父本染色体的分离。在这里，我们筛选的因素所需的建立单极附着在裂变酵母，并确定了一种新的蛋白质，Moa 1。Moa 1是减数分裂特异性的，并且专门定位于着丝粒的中央核心，该区域结合减数分裂的含有Rec 8的粘附素复合物，但不结合有丝分裂的含有Rad 21/Scc 1的复合物。在中央核心区域的Rec 8的强制裂解导致单极附着的破坏，如在Moa 1 Delta细胞中，而不减少Moa 1定位。Moa 1与Rec 8相互作用，这意味着Moa 1仅通过Rec 8发挥作用，可能是为了促进中央核心凝聚力。这些结果证明，单取向的着丝粒是建立在一个凝聚力介导的方式。
Meiosis resembles mitosis but employs a unique "reductional" nuclear division to allow the production of haploid gametes from diploid cells. The crucial ploidy reduction step requires that sister kinetochores attach to microtubules emanating from the same spindle pole, achieving "monopolar attachment," which ensures that maternal and paternal chromosomes are segregated. Here we screened for factors required to establish monopolar attachment in fission yeast and identified a novel protein, Moa1. Moa1 is meiosis specific and localizes exclusively to the central core of the centromere, a region that binds meiotic Rec8-containing cohesin complexes but not mitotic Rad21/Scc1-containing complexes. Enforced cleavage of Rec8 in the central core region led to the disruption of monopolar attachment, as in moa1 Delta cells, without diminishing Moa1 localization. Moa1 physically interacts with Rec8, implying that Moa1 functions only through Rec8, presumably to facilitate central core cohesion. These results prove that monoorientation of kinetochores is established in a cohesion-mediated manner.