TORC2 and the AGC kinase Gad8 regulate phosphorylation of the ribosomal protein S6 in fission yeast.
TORC2 and the AGC kinase Gad8 regulate phosphorylation of the ribosomal protein S6 in fission yeast.
复制标题
DOI：
10.1242/bio.20122022
复制
发表时间：
2012-09-15
期刊：
影响因子：
2.4
通讯作者：
Petersen J
中科院分区：
文献类型：
--
作者：
Du W;Hálová L;Kirkham S;Atkin J;Petersen J
TOR （Rapamycin Target Of Rapamycin）信号协调细胞生长和分裂，以响应细胞营养环境的变化。TOR激酶形成两种不同的复合物：TORC1和TORC2。在哺乳动物中，TORC1控制的S6K1激酶磷酸化核糖体蛋白S6，从而协调细胞大小和营养状况。我们发现裂糖菌AGC激酶Gad8与核糖体蛋白S6 （Rps6）共免疫沉淀，并调节其磷酸化状态。先前已经证明Gad8被TORC2磷酸化。与此一致，我们发现TORC2和TORC1调节Rps6的磷酸化。因此，分裂酵母中的S6磷酸化实际上代表了TORC1和TORC2组合活性的读出。相反，我们发现体内Maf1 （RNA聚合酶III的抑制因子）的磷酸化状态与TORC1活性特异性相关。
TOR (Target Of Rapamycin) signalling coordinates cell growth and division in response to changes in the nutritional environment of the cell. TOR kinases form two distinct complexes: TORC1 and TORC2. In mammals, the TORC1 controlled S6K1 kinase phosphorylates the ribosomal protein S6 thereby co-ordinating cell size and nutritional status. We show that the Schizosaccharomyces pombe AGC kinase Gad8 co-immunoprecipitates with the ribosomal protein S6 (Rps6) and regulates its phosphorylation status. It has previously been shown that Gad8 is phosphorylated by TORC2. Consistent with this, we find that TORC2 as well as TORC1 modulates Rps6 phosphorylation. Therefore, S6 phosphorylation in fission yeast actually represents a read-out of the combined activities of TORC1 and TORC2. In contrast, we find that the in vivo phosphorylation status of Maf1 (a repressor of RNA polymerase III) specifically correlates with TORC1 activity.
登录
查看更多内容
影响因子：
16
作者：
Loewith, R;Jacinto, E;Hall, MN
通讯作者：
Hall, MN
影响因子：
4
作者：
Nakashima, Akio;Sato, Tatsuhiro;Tamanoi, Fuyuhiko
通讯作者：
Tamanoi, Fuyuhiko
影响因子：
4.8
作者：
Smith, EM;Finn, SG;Proud, CG
通讯作者：
Proud, CG
影响因子：
2.1
作者：
Uritani, Masahiro;Hidaka, Hidetoshi;Toda, Takashi
通讯作者：
Toda, Takashi
影响因子：
5.3
作者：
Caspari, T;Dahlen, M;Carr, AM
通讯作者：
Carr, AM