Interplay between global and pathway-specific synaptic plasticity in CA1 pyramidal cells
Interplay between global and pathway-specific synaptic plasticity in CA1 pyramidal cells
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DOI：
10.1038/s41598-017-17161-z
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发表时间：
2017-12-06
影响因子：
4.6
通讯作者：
Koehr, Georg
中科院分区：
文献类型：
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作者：
Berberich, Sven;Pohle, Joerg;Koehr, Georg
信息存储的机制已经被描述为全局细胞范围和通路特异性突触可塑性。然而，很少有人知道这些形式的可塑性如何相互作用，以增强突触竞争和网络稳定性。我们研究了小鼠海马脑片CA 1区锥体神经元顶端和基底树突之间的突触相互作用。在成年小鼠中，与辐射层中的突触前刺激配对的三个动作电位（AP-爆发）的爆发（50 Hz）特异性地导致配对通路的LTP（P75）。在青春期（P28），爆发频率的增加（> 50 Hz）需要获得时间依赖性LTP。令人惊讶的是，配对的辐射和未配对的oriens路径增强，除非前后延迟从10 ms缩短到5 ms，这选择性地增强了配对的辐射路径，因为未配对的oriens路径降低到基线。相反，完全相同的5毫秒配对层oriens增强两个途径，单独的AP-突发，这增强了突触的功效，以及电流诱发的突触后尖峰。L-型电压门控钙通道参与介导的突触增强oriens，而NMDA和腺苷受体抵消未配对的oriens增强后，在放射层配对。这种不对称的可塑性揭示了重要的见解，传递海马和海马外信息的通路的突触功效和内在神经元兴奋性的改变。
Mechanisms underlying information storage have been depicted for global cell-wide and pathway-specific synaptic plasticity. Yet, little is known how these forms of plasticity interact to enhance synaptic competition and network stability. We examined synaptic interactions between apical and basal dendrites of CA1 pyramidal neurons in mouse hippocampal slices. Bursts (50 Hz) of three action potentials (AP-bursts) paired with preceding presynaptic stimulation in stratum radiatum specifically led to LTP of the paired pathway in adult mice (P75). At adolescence (P28), an increase in burst frequency (> 50 Hz) was required to gain timing-dependent LTP. Surprisingly, paired radiatum and unpaired oriens pathway potentiated, unless the pre-post delay was shortened from 10 to 5 ms, which selectively potentiated paired radiatum pathway, since unpaired oriens pathway decreased back to baseline. Conversely, the exact same 5 ms pairing in stratum oriens potentiated both pathways, as did AP-bursts alone, which potentiated synaptic efficacy as well as current-evoked postsynaptic spiking. L-type voltage-gated Ca2+ channels were involved in mediating synaptic potentiation in oriens, whereas NMDA and adenosine receptors counteracted unpaired stratum oriens potentiation following pairing in stratum radiatum. This asymmetric plasticity uncovers important insights into alterations of synaptic efficacy and intrinsic neuronal excitability for pathways that convey hippocampal and extra-hippocampal information.