Morphological changes of short-wavelength cones in the developing S334ter-3 transgenic rat.
Morphological changes of short-wavelength cones in the developing S334ter-3 transgenic rat.
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DOI：
10.1016/j.brainres.2010.01.051
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发表时间：
2010-03-19
期刊：
影响因子：
2.9
通讯作者：
Lee, Eun-Jin
中科院分区：
文献类型：
--
作者：
Hombrebueno, Jose R.;Tsai, Melody M.;Kim, Hong-Lim;De Juan, Joaquin;Grzywacz, Norberto M.;Lee, Eun-Jin
S334 ter-3大鼠是视网膜变性（RD）的转基因模型，其被开发用于表达类似于在人视网膜色素变性中发现的视紫红质突变。由于这一优势超过其他模型的RD，一些视网膜移植研究已经报告了这种动物模型。目前，没有关于S334 ter RD模型中发生的视锥光感受器变化的信息。在这项研究中，我们研究了RD对S334 ter-3大鼠视网膜发育过程中短波长锥（S-锥）的形态，分布和突触连接的影响。在P21 RD视网膜，外核层显着狭窄，而S-锥显示其节段和轴突相比，控制视网膜缩短。从P90开始，S-视蛋白免疫反应阳性细胞出现在RD视网膜内核层的外缘。双标记实验表明这些细胞含有recoverin和cone arrestin。超微结构观察显示，P180 RD视网膜S锥内的突触带较为保守。虽然细胞密度的S-锥显着下降后P90，生存率取决于视网膜区域。总体而言，S334 ter-3 RD模型显示出由于光感受器变性导致的视锥重塑的特征。
The S334ter-3 rat is a transgenic model of retinal degeneration (RD) developed to express a rhodopsin mutation similar to that found in human retinitis pigmentosa. Due to this advantage over other models of RD, a few retina transplant studies have been reported on this animal model. Currently, no information is available on cone photoreceptor changes that occur in the S334ter RD model. In this study, we investigated the effect of RD on the morphology, distribution, and synaptic connectivity of short-wavelength cones (S-cones) during development of S334ter-3 rat retinas. At P21 RD retinas, the outer-nuclear layer was significantly narrower, while S-cones showed shortening of their segments and axons compared to control retinas. From P90 onward, S-opsin-immunoreactive cells appeared at the outer margin of the inner-nuclear layer of RD retinas. Double-labelling experiments showed these cells contained recoverin and cone arrestin. Furthermore, ultra-structure study showed that synaptic ribbons are conserved in the S-cone at P180 RD retinas. Although cell density of S-cones significantly dropped after P90, survival rates depended on the retinal region. Overall, the S334ter-3 RD model shows hallmarks of cone remodelling due to photoreceptor degeneration.
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