Ecdysterone-sensitive smooth muscle cell proliferation stimulated by conditioned medium of endothelial cells cultured with bloody cerebrospinal fluid
Ecdysterone-sensitive smooth muscle cell proliferation stimulated by conditioned medium of endothelial cells cultured with bloody cerebrospinal fluid
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血性脑脊液培养内皮细胞条件培养基刺激蜕皮甾酮敏感平滑肌细胞增殖
DOI：
10.1007/978-3-211-75718-5_36
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Z. Liu
中科院分区：
文献类型：
--
作者：
J. H. Zhang;Z. Chen;冯华;G. Zhu;W. Tang,;Z. Liu
血管细胞增殖在脑血管痉挛发病机制中起重要作用的概念主要基于动物模型，并且仍然存在争议。为验证内皮细胞损伤可刺激血管平滑肌细胞（VSMCs）增殖及蜕皮甾酮（ECs）对VSMCs增殖的抑制作用，本实验采用含血脑脊液（BCSF）的培养液培养兔脑微血管内皮细胞（BMVECs），含或不含蜕皮甾酮，浓度为5 × 10− 5 mol/l。收集所得条件培养基（CM），分别采用Griess反应和放射免疫法测定一氧化氮（NO）和内皮素（ET-1）的含量。采用~ 3 H-胸腺嘧啶核苷掺入法和流式细胞术检测条件培养液对兔血管平滑肌细胞增殖的影响。条件培养基从血液脑脊液治疗刺激血管平滑肌细胞增殖比未经处理的培养基，以及条件培养基从血液脑脊液和蜕皮甾酮共治疗。结果表明，血脑脊液培养的脑微血管内皮细胞培养液可促进血管平滑肌细胞增殖，蜕皮甾酮可抑制这种增殖作用。
The concept that vascular cell proliferation plays an important role in the pathogenesis of cerebral vasospasm is based mainly on animal models and remains controversial. The objective of this study was to test the hypothesis that injured endothelial cells could stimulate the proliferation of vascular smooth muscle cells (VSMCs) and ecdysterone, a main active component of some traditional Chinese herbs, could play an inhibitory role in the process.Rabbit brain microvessel endothelial cells (BMVECs) were cultured with media containing bloody cerebrospinal fluid (BCSF), with or without ecdysterone at 5 × 10−5mol/l. The resulting conditioned media (CM) were collected and the content of nitric oxide (NO) and endothelin (ET-1) were determined by Griess reaction and radioimmunoassay, respectively. The proliferative effect of the conditioned media on rabbit vascular smooth muscle cells was evaluated by 3H-thymidine incorporation and flow cytometry analysis. Conditioned medium from bloody cerebrospinal fluid treatment stimulated more proliferation of vascular smooth muscle cells than the untreated medium as well as the conditioned medium from bloody CSF and ecdysterone co-treatment. This increased proliferation correlated with a decreased concentration of NO and an elevated concentration of ET-1 in the conditioned media.In conclusion, we observed that the medium from brain microvessel endothelial cells cultured with bloody cerebrospinal fluid activated vascular smooth muscle cell proliferationin vitroand the proliferative effect could be reduced by ecdysterone.
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影响因子：
4.1
作者：
FOLEY, PL;TAKENAKA, K;LEE, KS
通讯作者：
LEE, KS
影响因子：
4.8
作者：
Youssef G. Comair;Youssef G. Comair;Hyman M. Schipper;Steven Brem;Steven Brem
通讯作者：
Youssef G. Comair;Youssef G. Comair;Hyman M. Schipper;Steven Brem;Steven Brem
DOI：
10.1016/s0021-9258(19)38651-x
发表时间：
1993-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Grobmyer;A. Kuo;M. W. Orishimo;S. S. Okada-S.;D. Cines;E. Barnathan
通讯作者：
S. Grobmyer;A. Kuo;M. W. Orishimo;S. S. Okada-S.;D. Cines;E. Barnathan
DOI：
10.1161/01.str.0000033931.62992.b1
发表时间：
2002-11
期刊：
Stroke: Journal of the American Heart Association
影响因子：
--
作者：
P. Gabikian;R. Clatterbuck;C. Eberhart;B. Tyler;T. Tierney;R. Tamargo
通讯作者：
P. Gabikian;R. Clatterbuck;C. Eberhart;B. Tyler;T. Tierney;R. Tamargo
影响因子：
6.1
作者：
Chen, Q;Xia, YP;Qiu, ZY
通讯作者：
Qiu, ZY