Sandaracinobacteroides hominis gen. nov., sp. nov., isolated from human skin
Sandaracinobacteroides hominis gen. nov., sp. nov., isolated from human skin
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DOI：
10.1007/s00203-021-02454-9
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发表时间：
2021-07
影响因子：
2.8
通讯作者：
Pinghua Qu;Hai-min Luo;Jun-hui Feng;Song Li;Cha Chen;Lei Dong;Yu-Zhen Ming;Wenjun Li;Yingzhi Lin
中科院分区：
文献类型：
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作者：
Pinghua Qu;Hai-min Luo;Jun-hui Feng;Song Li;Cha Chen;Lei Dong;Yu-Zhen Ming;Wenjun Li;Yingzhi Lin
SZY PN-1 T菌株是从中国健康男性皮肤标本中分离到的一株革兰氏阴性、需氧、非运动、杆状、黄色的细菌。在pH 6.0-8.0（最适pH 7.0）和10-37 ℃（最适温度30 ℃）下，在R2 A琼脂中加入0-1.0%（w/v）NaCl时生长。16 S rRNA基因序列分析表明，SZY PN-1 T与已发表的两个失效种“西伯利亚山达喇琴鱼”RB 16 -17（97.1%）和“新斯坦山达喇琴鱼”JCM 30734（96.6%）具有较高的相似性。16 S rRNA基因序列的系统发育分析和蛋白质串联体树分析表明，SZY PN-1 T在Sphingosinicellaceae中形成了一个独立的分支。SZY PN-1 T的DNA G + C含量为65.0%（基因组）。极性脂质特征包括磷脂酰乙醇胺、磷脂酰甘油、两种鞘糖脂、二磷脂酰甘油、五种未鉴别糖脂和七种未鉴别脂质。主要脂肪酸（> 10.0%）被鉴定为C18：1ω 7 c和/或C18：1ω6c、C17：1ω6c、C16：1ω 7 c和/或C16：1ω6c。主要呼吸醌是Q-10。根据表型和基因型特征，将其命名为一个新属、新种-人山达杆菌属（Sandaracinobacteroides hominisgen）。十一月，nov.，模式菌株为SZY PN-1 T（= KCTC 82150 T = NBRC 114675 T）。
Strain SZY PN-1T, representing a novel Gram-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, was isolated from a skin sample of a healthy Chinese male. Growth occurred at pH 6.0–8.0 (optimum, pH 7.0) and 10–37 ℃ (optimum, 30 ℃) with 0–1.0% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that strain SZY PN-1Tshared high similarities with two invalid-published species, “Sandaracinobacter sibiricus” RB16-17 (97.1%) and “Sandaracinobacter neustonicus” JCM 30734 (96.6%), respectively. Phylogenetic analysis of 16S rRNA gene sequences together with protein-concatemer tree showed that SZY PN-1Tformed a separate branch within the familySphingosinicellaceae. The DNA G + C content of the strain SZY PN-1Twas 65.0% (genome). The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, two sphingoglycolipids, diphosphatidylglycerol, five unidentified glycolipids, and seven unidentified lipids. The predominant fatty acids (> 10.0%) were identified as C18:1ω7cand/or C18:1ω6c, C17:1ω6c, C16:1ω7cand/or C16:1ω6c. The major respiratory quinone was Q-10. Based on the phenotypic and genotypic features, a novel genus and species,Sandaracinobacteroides hominisgen. nov., sp. nov. is proposed, with type strain SZY PN-1T(= KCTC 82150T= NBRC 114675T).