RESPIRATORY SYNCYTIAL VIRUS ALTERS ELECTROPHYSIOLOGIC PROPERTIES IN COTTON RAT AIRWAY EPITHELIUM
RESPIRATORY SYNCYTIAL VIRUS ALTERS ELECTROPHYSIOLOGIC PROPERTIES IN COTTON RAT AIRWAY EPITHELIUM
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DOI：
10.1002/ppul.1950060307
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发表时间：
1989-01-01
影响因子：
3.1
通讯作者：
OGRA, PL
中科院分区：
文献类型：
--
作者：
CLOUTIER, MM;WONG, D;OGRA, PL
本文研究了呼吸道合胞病毒（RSV）感染对棉花大鼠气管在体内暴露后气道上皮电生理特性的影响。通过组织培养感染和免疫荧光抗体技术证实了RSV感染。暴露于RSV 72小时后的气管光镜研究显示上皮形态正常。RSV感染使短路电流（Isc）降低28%，组织电阻几乎增加100%。Isc的减少部分归因于CI分泌的减少，而组织阻力的增加与RSV感染气管中钠和氯化物通过细胞旁途径的显著限制有关。这些研究表明，RSV感染棉大鼠气管后，其主动和被动离子流动发生了显著变化。气道上皮离子转运的改变可能导致气道继发性水转运的改变，并可能导致儿童RSV细支气管炎和其他局部侵袭性粘膜病毒感染的病理生理。
The effect of respiratory syncytial virus (RSV) infection on the electrophysiologic properties of the airway epithelium was studied in tracheas obtained from cotton rats, after in vivo exposure to the virus. RSV infection was documented by tissue culture infectivity and immunofluorescent antibody techniques. Light microscopic studies of the tracheas 72 hours after exposure to RSV revealed normal epithelial morphology. RSV infection produced a 28% decrease in short‐circuit current (Isc) and an almost 100% increase in tissue resistance. The decrease in Isc was partially attributed to a decrease in CI secretion, while the increase in tissue resistance was associated with significant restriction to sodium and chloride movement through the paracellular pathway in RSV‐infected tracheas. We conclude from these studies that RSV infection in the cotton rat trachea produces significant changes in active and passive ion flows. Alterations in ion transport across the airway epithelium may result in changes in secondary water transport across the airways and may contribute to the pathophysiology of RSV bronchiolitis and other locally invasive mucosal viral infections in children.