Elevated expression of G protein-coupled receptor 30 (GPR30) is associated with poor prognosis in patients with uterine cervical adenocarcinoma
Elevated expression of G protein-coupled receptor 30 (GPR30) is associated with poor prognosis in patients with uterine cervical adenocarcinoma
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DOI：
10.14670/hh-18-157
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发表时间：
2020-04-01
影响因子：
2
通讯作者：
Osanai, Makoto
中科院分区：
文献类型：
--
作者：
Ino, Yoshihiko;Akimoto, Taishi;Osanai, Makoto
子宫腺癌的预后比鳞癌差，需要有用的诊断和预后标记物。雌激素是多种癌症的关键调节因子之一，然而，雌激素信号在宫颈腺癌中的作用尚未引起人们的关注。在这里，我们显示了经典的雌激素受体(ER)和一种新的膜型雌激素受体G蛋白偶联受体30(GPR30)在手术标本中的表达情况(n=53)。GPR30在原位腺癌和腺癌的细胞膜和胞浆中均有较强的表达，在大多数GPR30阳性的腺癌中，其在侵袭前沿的表达尤其强。ER在非肿瘤性腺体中表达较强，而在大多数AIS和腺癌中几乎不表达。宫颈鳞癌和腺癌组织中GPR30和ER的表达呈微弱的负相关(r=0.324，p=0.017)。ROC曲线分析显示，GPR30免疫反应能成功区分肿瘤和非肿瘤性腺体，特异性(100%)和敏感性(75.5%)。GPR30阳性表达与组织学类型(p=0.009)、肿瘤直径(p=0.003)、肿瘤大小(p
Uterine cervical adenocarcinoma has a worse prognosis than that of squamous cell carcinoma and useful diagnostic and prognostic markers are needed. Estrogen is one of the key regulators of several cancers, however, the estrogen signaling has not been focused on in cervical adenocarcinoma. Here, we shows expression profile of classical estrogen receptor (ER) and a novel membrane type estrogen receptor, G protein-coupled receptor 30 (GPR30), in surgical specimens (n=53). GPR30 was strongly expressed on the cell membrane and in the cytoplasm in adenocarcinoma in situ (AIS) and adenocarcinoma, and its expression was especially strong at the invasion front in most of the cases of GPR30-positive adenocarcinoma. Nuclear staining of ER was strong in non-neoplastic glands, whereas it was almost absent in most of the AIS and adenocarcinoma cases. There was a weak but statistically significant negative correlation between immunoreactivity of GPR30 and that of ER in cervical AIS and adenocarcinoma lesions (Spearman's correlation, r=0.324, p=0.017). ROC curve analysis revealed that immunoreactivity of GPR30 successfully distinguished neoplasms from non-neoplastic glands with high specificity (100%) and sensitivity (75.5%). GPR30 positivity was significantly correlated with histological type (p=0.009), tumor diameter (p=0.003), tumor size (p