HISTOCHEMICAL METHODS FOR CALCIUM
HISTOCHEMICAL METHODS FOR CALCIUM
复制标题
DOI：
10.1177/6.1.22
复制
发表时间：
1958-01-01
影响因子：
3.2
通讯作者：
MCGEERUSSELL, SM
中科院分区：
文献类型：
--
作者：
MCGEERUSSELL, SM
在测试组织中给出可重复和一致结果的方法是金属替代试验或染料湖反应。可以用银、钴、铁和亚铁、铅和铜盐实现满意的工厂金属替代。在组织学切片上与钙沉积物发生良好湖反应的蒽醌染料有：芹素磺酸钠、1:2:4-三羟基蒽醌、紫癜-3-羧酸、1:2:5:8-四羟基蒽醌和钙红（即kernechtrot）。在特殊情况下，血红素可与钙发生有效的湖反应。在动物体内，许多钙沉积物可以很好地用试剂rhodizonate定位，显然，如果沉积物被钡或锶污染。胆碱蓝，一种被推荐用于钙染色的恶嗪染料，作为钙试剂没有一定的组织化学有效性。为了建立组织中钙定位的组织化学有效性，有必要采用所有成功的方法。
Methods giving reproducible and consistent results in test tissues are either metal substitution tests or dye-lake reactions. Satis- factory metal substitution can be achieved with silver, cobalt, ferric and ferrous iron, lead, and copper salts. Anthraquinone dyes which produce good lake reactions with calcium deposits in histological sections are: sodium alizarin sulfonate, 1:2:4-trihydroxyanthraquinone, purpurin-3-carboxylic acid, 1:2:5:8-tetrahydroxyanthraqulnone, and calcium red (i.e., kernechtrot). Haematoxylin can produce a valid lake reaction with calcium, under special circumstances. In animals, many calcium deposits can be very well localized with the reagent sodium rhodizonate, apparently if the deposits are contaminated with barium or strontium. Gallamine blue, an oxazin dyestuff which has been recommended for calcium staining, has no certain histochemical validity as a calcium reagent. To establish full histochemical validity for a site of localization of calcium in a tissue it is necessary to employ all the successful methods.