Identification of a novel cognate cytosolic Hsp70 gene (MnHsc70-2) from oriental river prawn Macrobrachium nipponense and comparison of its expressions with the first cognate Hsc70 (MnHsc70-1) under different stresses
Identification of a novel cognate cytosolic Hsp70 gene (MnHsc70-2) from oriental river prawn Macrobrachium nipponense and comparison of its expressions with the first cognate Hsc70 (MnHsc70-1) under different stresses
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日本沼虾新型同源胞质 Hsp70 基因 (MnHsc70-2) 的鉴定及其与第一个同源 Hsc70 (MnHsc70-1) 在不同胁迫下的表达比较
DOI：
10.1007/s12192-014-0519-2
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Ren Qian;Gu Wei;Meng Qingguo;Wang, Wen
热休克蛋白70（Hsp 70）是一个分子量为70 kDa的热休克蛋白家族，在真核细胞中广泛表达，是一种重要的分子伴侣蛋白，在宿主免疫中起重要作用。在本研究中，全长互补DNA（cDNA）的第二个同源胞质Hsp 70家族成员（MnHsc 70 -2）的克隆和表征日本沼虾，这是一个经济和营养重要的甲壳动物。该cDNA全长2,717 bp，含有1 950 bp的开放阅读框（ORF），编码649个氨基酸，理论分子量为71.1 kDa，等电点为5.27。序列比对表明，MnHsc 70 -2与其它热休克蛋白的同源性为75- 97%。与先前在M. MnHsc 70 -2在非胁迫条件下，在血细胞、心脏、肝胰腺、鳃、肠、神经和肌肉等组织中的表达谱存在显著差异。系统发育分析表明，MnHsc 70 -2与MnHsc 70 -1的亲缘关系最近。热诱导实验表明，两个分离的信使RNA（mRNA）显示不同的表达谱在肝胰腺和鳃组织。MnHsc 70 -1 mRNA表达水平呈先下降后上升趋势，MnHsc 70 -2 mRNA表达水平呈先上升后下降趋势。两个MnHsc 70在肝胰腺和鳃中的表达均表现出明显的热诱导调控。在嗜水气单胞菌（Aeromonasseriae）的攻击下，MnHsc 70 -1和MnHsc 70 -2的mRNA水平均适度上调。结果表明，两个同源Hsc 70可能在介导热休克和细菌攻击的反应中发挥重要作用。
The 70-kDa family of heat-shock proteins (Hsp70) plays an important role in the host immunity, which is widely expressed in eukaryotic cells as a major chaperone protein. In the present study, the full-length complementary DNA (cDNA) of a second cognate cytosolic Hsp70 family member (MnHsc70-2) was cloned and characterized from Macrobrachium nipponense, which is an economically and nutritionally important crustacean. The cDNA was 2,717 bp, containing an open reading frame (ORF) of 1,950 bp, which encodes a protein of 649 amino acids with a theoretical molecular weight of 71.1 kDa and an isoelectric point of 5.27. Sequence alignment showed that the MnHsc70-2 shared 75-97 % identity with other heat-shock proteins. Compared to the previously identified cognate Hsp70 (MnHsc70-1) in M. nipponense, MnHsc70-2 showed quite different expression profiles under unstressed conditions in all tested tissues, including the hemocytes, heart, hepatopancreas, gill, intestine, nerve, and muscle. The phylogenetic analysis demonstrated that MnHsc70-2 showed the closest relationship with MnHsc70-1. Heat-inducibility assays showed that two isolated messenger RNAs (mRNAs) displayed different expression profiles in both the hepatopancreas and gill tissues. MnHsc70-1 mRNA expression level decreased at first and then increased to the normal level, whereas MnHsc70-2 mRNA level increased at first and then decreased. The expressions of two MnHsc70s showed substantial obvious heat-inducible regulation in both the hepatopancreas and gill. Under bacterial challenge by Aeromonas hydrophila, both MnHsc70-1 and MnHsc70-2 mRNA level was up-regulated moderately. The results suggested that two cognate Hsc70s may play essential functions in mediating responses to heat-shock and bacterial challenge.
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影响因子：
5.3
作者：
Demand, J;Lüders, J;Höhfeld, J
通讯作者：
Höhfeld, J
影响因子：
4.7
作者：
Ming, Jianhua;Xie, Jun;Pan, Liangkun
通讯作者：
Pan, Liangkun
影响因子：
1.2
作者：
R. Wu;Y. Sun;L. Lei;S. Xie
通讯作者：
R. Wu;Y. Sun;L. Lei;S. Xie
影响因子：
4.8
作者：
Johnson, BD;Schumacher, RJ;Toft, DO
通讯作者：
Toft, DO
DOI：
10.1002/9781119432401.ch9
发表时间：
2020-02
期刊：
Heat Stress Tolerance in Plants
影响因子：
--
作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi
通讯作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi