Protective effects of tanshinone ⅡA on endothelial progenitor cells injured by tumor necrosis factor-α.
Protective effects of tanshinone ⅡA on endothelial progenitor cells injured by tumor necrosis factor-α.
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DOI：
10.3892/mmr.2015.3969
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发表时间：
2015-09
影响因子：
3.4
通讯作者：
Zhang YF
中科院分区：
文献类型：
--
作者：
Wang XX;Yang JX;Pan YY;Zhang YF
丹参酮IIA(Tan IIA)是亚洲和西方国家常用的一种中药，用于预防和治疗心血管疾病，如动脉粥样硬化。内皮功能障碍和相关的炎症过程在动脉粥样硬化的发展中起着关键作用。内皮祖细胞(EPC)已被证明参与内皮修复过程的某些方面。本研究旨在探讨丹参IIA对肿瘤坏死因子-α(肿瘤坏死因子-α)损伤内皮祖细胞的保护作用。本研究观察了丹参IIA对肿瘤坏死因子-α刺激的内皮细胞增殖、迁移、黏附、体外成管能力和旁分泌功能的影响。结果表明，肿瘤坏死因子-α抑制内皮祖细胞的增殖、迁移、黏附能力和血管生成能力，促进其分泌炎性细胞因子，包括单核细胞趋化蛋白-1、白细胞介素6和可溶性CD40L。然而，Tan IIA能够逆转这些影响。综上所述，这些发现表明Tan IIA可能具有保护内皮祖细胞免受肿瘤坏死因子-α损伤的作用。因此，这些结果可能为丹参IIA的药理基础及其在防治EPC和内皮损伤相关的早期动脉粥样硬化中的潜在应用提供证据。
Tanshinone IIA (Tan IIA) is a Traditional Chinese Medicine commonly used in Asian and Western countries for the prevention and treatment of cardiovascular disorders, such as atherosclerosis. Endothelial dysfunction and associated inflammatory processes have a critical role in the development of atherosclerosis. Endothelial progenitor cells (EPCs) have been demonstrated to be involved in certain aspects of the endothelial repair process. The present study aimed to investigate the putative protective effects of Tan IIA on EPCs injured by tumor necrosis factor-α (TNF-α). The potential effects of Tan IIA on TNF-α-stimulated EPC proliferation, migration, adhesion, in vitro tube formation ability and paracrine activity were investigated in the current study. The results indicated that TNF-α impaired EPC proliferation, migration, adhesion capacity and vasculogenesis ability in vitro as well as promoted EPC secretion of inflammatory cytokines, including monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and soluble CD40 ligand (sCD40L). However, Tan IIA was able to reverse these effects. In conclusion, these findings demonstrated that Tan IIA may have the potential to protect EPCs against damage induced by TNF-α. Therefore, these results may provide evidence for the pharmacological basis of Tan IIA and its potential use in the prevention and treatment of early atherosclerosis associated with EPC and endothelial damage.