Myoglobin oxygenation remains constant during the cardiac cycle.
Myoglobin oxygenation remains constant during the cardiac cycle.
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肌红蛋白氧合在心动周期中保持恒定。
DOI：
10.1152/ajpheart.1983.245.2.h237
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
M. Nakamura
中科院分区：
文献类型：
--
作者：
N. Makino;H. Kanaide;R. Yoshimura;M. Nakamura
在Langendorff法灌注的离体大鼠心脏中，采用光纤分光光度法记录心肌周期中肌红蛋白（Mb）的氧饱和度。通过左心室游离壁在581-592 nm处透射光的吸光度增量连续测量Mb的氧饱和度。此外，通过568-592 nm处的吸光度变化，即两个等吸点的双波长，定量评价心周期壁厚变化引起的Mb氧饱和度。结果表明，要得到实际的Mb氧饱和度，必须从581 ~ 592 nm的吸光度变化中减去由壁厚变化引起的吸光度变化，而稳定状态下的Mb氧饱和度决定了减去的量。在这些程序的基础上，我们发现在有氧和无氧稳定状态下搏动时心肌Mb氧饱和度和心肌氧张力在心脏周期中没有变化。
Oxygen saturation of myoglobin (Mb) during the cardiac cycle was recorded spectrophotometrically by incorporating fiber optics in the isolated rat heart perfused using the Langendorff procedure. Oxygen saturation of Mb was continuously measured from absorbancy increments at 581-592 nm of transmitted light through the left ventricular free wall. In addition, quantification in the Mb oxygen saturation induced by the change of wall thickness during cardiac cycle was assessed from the absorbance change at 568-592 nm, namely, dual wavelengths of two isosbestic points. The results show that to obtain actual Mb oxygen saturation the absorbance change induced by the change in the wall thickness has to be subtracted from the absorbance change of 581-592 nm and that the Mb oxygen saturation in a steady state determines the amount of subtraction. On the basis of these procedures, it was found that the myocardial Mb oxygen saturation and hence myocardial oxygen tension during pulsation in aerobic and anaerobic steady state did not vary during the cardiac cycle.