Cytotoxicity of glutaraldehyde crosslinked collagen/poly(vinyl alcohol) films is by the mechanism of apoptosis
Cytotoxicity of glutaraldehyde crosslinked collagen/poly(vinyl alcohol) films is by the mechanism of apoptosis
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DOI：
10.1002/jbm.10145
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发表时间：
2002-07-01
期刊：
影响因子：
--
通讯作者：
Downes, S
中科院分区：
文献类型：
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作者：
Gough, JE;Scotchford, CA;Downes, S
胶原蛋白由于存在于细胞外基质中而被研究为潜在的天然生物材料。在这种情况下，胶原蛋白需要通过通常具有细胞毒性的试剂进行交联。戊二醛是一种具有潜在细胞毒性的此类试剂。本研究的目的是确定胶原蛋白/聚乙烯醇生物人工复合膜，当与戊二醛交联时，差的细胞附着和生长的原因。使用脱水热交联作为比较。观察到人成骨细胞在戊二醛交联膜上发生凋亡，这取决于胶原蛋白的浓度。较高的胶原含量导致较高水平的细胞凋亡，细胞附着较差，剩余细胞扩散。用8%L-谷氨酸后处理的膜防止成骨细胞的凋亡反应，并允许附着和扩散。向培养基中加入100 nM胰岛素样生长因子-1也可防止细胞凋亡。交联胶原的戊二醛毒性在本研究中已经被证实，其机制是细胞凋亡。这项研究表明，差的生物相容性和诱导细胞凋亡的胶原蛋白/聚（乙烯醇）膜交联的戊二醛是由于膜表面的戊二醛成分（而不是残留的戊二醛），其效果可以被谷氨酸淬灭，并防止胰岛素样生长因子-1。(C)2002 Wiley Periodicals，Inc.
Collagen has been investigated as a potential natural biomaterial, because of its occurrence in the extracellular matrix. Collagen requires crosslinking in this context, by reagents that are often cytotoxic. Glutaraldehyde is one such agent that is potentially cytotoxic. The aim of this study was to determine the cause of poor cell attachment and growth on collagen/poly(vinyl alcohol) bioartificial composite films, when crosslinked with glutaraldehyde. Dehydrothermal crosslinking was used as a comparison. Human osteoblasts were observed to undergo apoptosis on glutaraldehyde crosslinked films dependent on concentration of collagen present. Higher collagen content resulted in higher levels of apoptosis with poor cell attachment and spreading of remaining cells. Post-treatment of films with 8% L-glutamic acid prevented the apoptotic response of osteoblasts and allowed attachment and spreading. The addition of 100 nM insulin-like growth factor-1 to the Culture medium also prevented apoptosis. Glutaraldehyde toxicity of crosslinked collagen has been demonstrated in this study, the mechanism of which is apoptosis. This study indicates that poor biocompatibility and induction of apoptosis on collagen/poly(vinyl alcohol) films crosslinked by glutaraldehyde are attributed to glutaraldehyde components on the surface of the films (not residual glutaraldehyde), whose effects can be quenched by glutamic acid, and prevented by insulin-like growth factor-1. (C) 2002 Wiley Periodicals, Inc.