Derivatization-based High-throughput Bioanalysis by LC-MS
Derivatization-based High-throughput Bioanalysis by LC-MS
复制标题
DOI：
10.2116/analsci.33.555
复制
发表时间：
2017-05
影响因子：
1.6
通讯作者：
T. Toyo′oka
中科院分区：
文献类型：
--
作者：
T. Toyo′oka
液相色谱-质谱联用（LC-MS）由于其固有的选择性和灵敏度而成为最突出的分析技术之一。由于质谱仪器和分析软件的进步，LC-MS是目前高通量生物分析的首选。基于这种情况，我们正在开发各种类型的衍生试剂，包括用于MS和/或MS/MS检测的手性试剂。这些开发的试剂用于检测与疾病相关的生物标志物候选物。生物标志物候选物不仅包括非手性分子，而且包括手性分子。虽然确定已经鉴定的手性分子相对容易，但鉴定和/或确定真实的样品中的未知对映异构体是非常困难的。为了解决这一难题，我们提出了一种新的策略来识别未知的与疾病相关的生物标记物。本文综述了LC-MS分析中胺类和羧酸类衍生试剂的发展及其在生物分析中的应用。
Liquid chromatography coupled with mass spectrometry (LC-MS) is one of the most prominent analytical techniques due to its inherent selectivity and sensitivity. LC-MS is currently the first choice for high-throughput bioanalysis due to the advancements in MS instruments and the analytical software. Based on this situation, we are developing various types of derivatization reagents, including chiral reagents for MS and/or MS/MS detection. These developed reagents are adopted for the detection of biomarker candidates related to diseases. The biomarker candidates include not only achiral molecules, but also chiral ones. Although determining the already-identified chiral molecules is relative easy, it is very difficult to identify and/or determine unknown enantiomer(s) in real samples. To solve this difficulty, we proposed a new strategy to identify unknown enantiomeric biomarkers related to diseases. This review paper deals with the development of derivatization reagents for amines and carboxylic acids in LC-MS analysis and their application to bioanalysis.