Exploring the potential of microcalorimetry to study soil microbial metabolic diversity
Exploring the potential of microcalorimetry to study soil microbial metabolic diversity
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DOI：
10.1007/s10973-016-5952-2
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发表时间：
2017-02
影响因子：
4.4
通讯作者：
Jiangbing Xu;Youzhi Feng;N. Barros;L. Zhong;Ruirui Chen;Xiangui Lin
中科院分区：
文献类型：
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作者：
Jiangbing Xu;Youzhi Feng;N. Barros;L. Zhong;Ruirui Chen;Xiangui Lin
微量热法和Biolog是研究土壤微生物代谢的常用工具。当它们结合使用时，它们可能比单独使用更能揭示土壤微生物代谢多样性的细节。通过这项研究，我们通过对中国两个地点的土壤样本进行比较，证明了这种组合方法的优势，每个地点都有(OM样本)和没有(对照)有机肥。我们使用Biolog和微量热法研究了土壤微生物对不同C底物的代谢能力。微量热测量帮助我们进一步揭示了不同Biolog确定的C底物下微生物生长动力学的差异。结果表明，根据热力学参数的不同，不同土壤具有不同的优先碳基质。一些C底物促进了微生物的活性生物量，而另一些则促进了微生物的生长速度。最有趣的是，某些C底物(如山东土壤的L-精氨酸和河南土壤的糖原)对有机质和对照土壤都表现出刺激作用，这可以归因于土壤的pH值和磷的有效性。因此，我们相信微量热法可以通过结合Biolog测量来探索土壤微生物代谢多样性，特别是在确定微生物如何同化不同的营养来源方面。
Microcalorimetry and BIOLOG are common tools in the study of soil microbial metabolism. When used combined, they may reveal further details about soil microbial metabolic diversity than individually. Through this study, we demonstrated the advantages of such a combinatorial methodology by comparing soil samples from two locations in China, each with (OM samples) and without (control) organic fertilization. We used BIOLOG and microcalorimetry to study soil microbes’ ability to metabolize different C substrates. Microcalorimetric measurements helped us further reveal the differences in the microbial growth kinetics under different BIOLOG-identified C substrates. Results showed that soils differed in the preferred C substrates, as denoted by the thermodynamic parameters. Some C substrates stimulated the active microbial biomass, while some stimulated microbial growth rate. Most interestingly, certain C substrates (e.g.,l-arginine for Shandong soil and glycogen for Henan soil) showed stimulating effects on both OM and control soils, which could be attributed to the pH value and P availability in soil. Hence, we believe microcalorimetry could be potentially used to explore the soil microbial metabolic diversity by combining BIOLOG measurement, especially in determining how microbes assimilate different nutrient sources.