Evaluation of a high-hardness COMBO medium and frozen algae for Daphnia magna
Evaluation of a high-hardness COMBO medium and frozen algae for Daphnia magna
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DOI：
10.1006/eesa.1997.1627
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发表时间：
1998-03-01
影响因子：
6.8
通讯作者：
Goulden, CE
中科院分区：
文献类型：
--
作者：
Baer, KN;Goulden, CE
对一种新的高硬度（H-H）COMBO培养基进行了21天的存活、生长和繁殖研究，以评价该培养基对大型蚤的长期培养和生物测定。最初设计用于软体水蚤物种的COMBO培养基（即，D. pulicaria）为硬水种D. magna.该培养基还允许连续培养水蚤的绿色藻类食物源，镰状锚丝藻。在H-H COMBO培养基中培养12代后，平均繁殖力为128+/-12（变异系数=9.4%）。在6个月的连续培养期间，未观察到死亡幼仔、流产卵或触角减少。参考毒物标准3，4-二氯苯胺（3，4-DCA）的无可见效应浓度（NOEC）为8.3微克/升，与公布的数值相似。这些研究表明，H-H COMBO是一种可接受的培养基，可用于培养和生物测定D.，magna使用A. falcatus进行。虽然平均生殖力（64+/-7）和成虫长度与以新鲜藻类为食的水蚤相比显著降低，但3，4-DCA的无观测效应浓度大于25微克/升。在生物测定测试中使用这种替代食物来源似乎很有希望，但需要进一步优化饲养率。(C)北京：科学出版社.
A new high-hardness (H-H) COMBO medium for long-term culturing and bioassay testing of Daphnia magna was evaluated in 21-day survival, growth, and reproduction studies. The COMBO medium originally designed for softwater daphnid species (i.e., D. pulicaria) was modified for the hardwater species D. magna. This medium also allowed continuous culturing of a green algal food source for daphnids, Ankistrodesmus falcatus. After 12 generations of culturing in H-H COMBO medium, the mean fecundity was 128+/-12 (coefficient of variation=9.4%). No significant observations of dead young, aborted eggs, or reduced antennas were made during the 6 months of continuous culturing. The no-observable-effect concentration (NOEC) for a reference toxicant standard, 3,4-dichloroaniline (3,4-DCA), was 8.3 mu g/liter, which is similar to published values. These studies demonstrate that H-H COMBO is an acceptable medium for use for culturing and bioassay testing with D., magna Additional experiments using frozen aliquots of A. falcatus were conducted. Although mean fecundity (64+/-7) and adult length were significantly decreased from the values for daphnids fed fresh algae, the NOEC for 3,4-DCA was >25 mu g/liter. The use of this alternative food source in bioassay testing appears promising, but further optimization of feeding rates is warranted. (C) 1998 Academic Press.