Rational Design of Heterodimeric Protein using Domain Swapping for Myoglobin
Rational Design of Heterodimeric Protein using Domain Swapping for Myoglobin
复制标题
使用肌红蛋白结构域交换合理设计异二聚体蛋白。
DOI：
10.1002/anie.201409267
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发表时间：
2015-01-07
影响因子：
16.6
通讯作者：
Hirota, Shun
中科院分区：
文献类型：
--
作者：
Lin, Ying-Wu;Nagao, Satoshi;Hirota, Shun
蛋白质设计是构建新型人工蛋白质的有效方法。提出了一种合理的方法，设计一个异源二聚体的蛋白质结构域交换马肌红蛋白（Mb）。通过X射线晶体学分析证实，从Mb的两个表面突变体有效地产生具有两个不同活性位点的异源二聚体Mb，其中参与二聚体盐桥的两个氨基酸的电荷在每个突变体中被单独反转，其中一个突变体的活性位点被修饰。本研究表明，通过结构域交换构建异源二聚体Mb的方法对于设计人工多血红素蛋白是有用的。
Protein design is a useful method to create novel artificial proteins. A rational approach to design a heterodimeric protein using domain swapping for horse myoglobin (Mb) was developed. As confirmed by X-ray crystallographic analysis, a heterodimeric Mb with two different active sites was produced efficiently from two surface mutants of Mb, in which the charges of two amino acids involved in the dimer salt bridges were reversed in each mutant individually, with the active site of one mutant modified. This study shows that the method of constructing heterodimeric Mb with domain swapping is useful for designing artificial multiheme proteins.