tie2, a putative protein tyrosine kinase from a new class of cell surface receptor.
tie2, a putative protein tyrosine kinase from a new class of cell surface receptor.
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tie2，一种来自新型细胞表面受体的推定蛋白酪氨酸激酶。
作者：
A. Runting;Steven A. Stacker;Andrew F. Wilks
从小鼠肺文库中分离到一种新的小鼠细胞表面受体（tie 2）的cDNA。预测的tie 2的氨基酸序列编码1122个氨基酸的蛋白质，具有由跨膜区平分的细胞外结构域和细胞内酪氨酸激酶结构域。胞外结构域由两个Ig样结构域、三个富含半胱氨酸的结构域和三个纤连蛋白III型重复序列组成，而胞内酪氨酸激酶结构域具有15个氨基酸的短插入区。在体外转录/翻译的tie 2 cDNA表明，它编码的糖蛋白的一些145 kDa。tie 2蛋白表现出与细胞表面受体tie的高度相似性（Partanen，J.（1992）Mol. Cell.生物学：12，1698-1707），并且与该蛋白质一起定义了一类新的细胞表面受体。
The cDNA of a novel mouse cell surface receptor (tie2) has been isolated from a mouse lung library. The predicted amino acid sequence of tie2 encodes a protein of 1122 amino acids, with an extracellular domain and an intracellular tyrosine kinase domain bisected by a transmembrane region. The extracellular domain consists of two Ig-like domains, three cysteine-rich domains and three fibronectin type III repeats whilst the intracellular tyrosine kinase domain has a short insert region of 15 amino acids. In vitro transcription/translation of the tie2 cDNA demonstrates that it encodes a glycoprotein of some 145 kDa. The tie2 protein exhibits a high degree of similarity to the cell surface receptor tie, (Partanen, J. et al., (1992) Mol. Cell. Biol., 12, 1698-1707), and together with this protein defines a new class of cell surface receptor.