The structure and nucleotide sequence of the 5' end of the human c-myc oncogene.
The structure and nucleotide sequence of the 5' end of the human c-myc oncogene.
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DOI：
10.1073/pnas.80.20.6307
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发表时间：
1983-10
影响因子：
11.1
通讯作者：
R. Watt;K. Nishikura;J. Sorrentino;A. Ar-rushdi;C. Croce;G. Rovera
中科院分区：
文献类型：
--
作者：
R. Watt;K. Nishikura;J. Sorrentino;A. Ar-rushdi;C. Croce;G. Rovera
我们利用从胎儿肝脏文库中分离的克隆基因组片段（克隆lambda MC41）和从人白血病细胞系K562中克隆的cDNA，建立了人c-myc癌基因5'端的结构和核苷酸序列。人类c-myc癌基因由三个外显子和两个内含子组成。三种不同细胞系的人c-myc mRNA引物延伸和S1核酸酶保护实验确定了两个转录起始位点的位置。通过对基因组和cDNA克隆序列的比较分析，确定了第一外显子-内含子边界的剪接位点。第一个外显子在所有三个阅读框中都包含终止密码子，没有翻译起始信号，证实了我们之前的观察，即c-myc mRNA具有长5'非编码序列。这个第一个外显子也被发现在多种人类细胞系中用于形成c-myc mrna。
We have established the structure and nucleotide sequence of the 5' end of the human c-myc oncogene, using a cloned genomic fragment isolated from a fetal liver library (clone lambda MC41) and cloned cDNA from the human leukemic cell line K562. The human c-myc oncogene consists of three exons and two introns. Primer extension of the human c-myc mRNA of three different cell lines and S1 nuclease protection experiments served to establish the position of two transcription initiation sites. The splicing site of the first exon-intron boundary was determined by comparative analysis of the sequences of the genomic and cDNA clones. The first exon contains termination codons in all three reading frames and no translation initiation signals, confirming our previous observation that the c-myc mRNA has a long 5' noncoding sequence. This first exon also was found to be utilized in the formation of c-myc mRNAs in a variety of human cell lines.