Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines
Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines
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DOI：
10.1016/j.nutres.2013.07.009
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发表时间：
2013-10-01
影响因子：
4.5
通讯作者：
Schroten, Horst
中科院分区：
文献类型：
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作者：
Weichert, Stefan;Jennewein, Stefan;Schroten, Horst
母乳低聚糖有助于预防母乳喂养婴儿的传染病。由于更复杂的寡糖的可用性缩短，更大规模的测试，特别是在动物模型和人类临床研究中，仍然受到限制。本研究的目的是评估全细胞生物催化合成的2'-岩藻糖基乳糖（2'-FL）和3-岩藻糖基乳糖（3-FL）的体外生物活性。因此，我们测试了这些寡糖在两种不同的人上皮细胞系中抑制病原体粘附的潜力。 2'-FL 可以抑制空肠弯曲杆菌、肠病性大肠杆菌、肠沙门氏菌和铜绿假单胞菌对肠道人细胞系 Caco-2 的粘附（分别减少 26%、18%、12% 和 17%），如 3-FL 所示（肠病性大肠杆菌 29%，P铜绿假单胞菌 26%）。此外，2'-FL和3-FL显着抑制铜绿假单胞菌对人呼吸道上皮细胞系A549的粘附（分别减少24%和23%）。这些结果证实了生物技术合成的母乳低聚糖的生物和功能活性。大规模定制的母乳低聚糖将来可用于补充婴儿配方奶粉成分或作为预防剂以减少传染病的影响。 (C) 2013 Elsevier Inc. 保留所有权利。
Human milk oligosaccharides help to prevent infectious diseases in breastfed infants. Larger scale testing, particularly in animal models and human clinical studies, is still limited due to shortened availability of more complex oligosaccharides. The purpose of this study was to evaluate 2'-fucosyllactose (2'-FL) and 3-fucosyllactose (3-FL) synthesized by whole-cell biocatalysis for their biological activity in vitro. Therefore, we have tested these oligosaccharides for their inhibitory potential of pathogen adhesion in two different human epithelial cell lines. 2'-FL could inhibit adhesion of Campylobacter jejuni, enteropathogenic Escherichia coli, Salmonella enterica serovar fyris, and Pseudomonas aeruginosa to the intestinal human cell line Caco-2 (reduction of 26%, 18%, 12%, and 17%, respectively), as could be shown for 3-FL (enteropathogenic E coli 29%, P aeruginosa 26%). Furthermore, adherence of P aeruginosa to the human respiratory epithelial cell line A549 was significantly inhibited by 2'-FL and 3-FL (reduction of 24% and 23%, respectively). These results confirm the biological and functional activity of biotechnologically synthesized human milk oligosaccharides. Mass-tailored human milk oligosaccharides could be used in the future to supplement infant formula ingredients or as preventatives to reduce the impact of infectious diseases. (C) 2013 Elsevier Inc. All rights reserved.