INTERNALLY QUENCHED FLUOROGENIC SUBSTRATE FOR FURIN
INTERNALLY QUENCHED FLUOROGENIC SUBSTRATE FOR FURIN
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DOI：
10.1006/abio.1995.1058
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发表时间：
1995-01-01
影响因子：
2.9
通讯作者：
THOMAS, G
中科院分区：
文献类型：
--
作者：
ANGLIKER, H;NEUMANN, U;THOMAS, G
合成了一种新型呋喃底物Abz-Arg-Val-Lys-Arg-Gly-Leu-Ala-Tyr(NO2)-Asp-OH，，并对其进行了表征。该肽是一种内部猝灭的荧光底物。动力学参数为：K-m=3.8u M，k(CAT)=29.3 S(-1)，k(CAT)/K-M=7,710,000 M(-1)S(-1)。底物被呋喃有效地切割，其k(CAT)/K-M值是常用底物Boc-Arg-Val-Arg-Arg-AMC的2000倍以上。(C)1995年学术出版社。
A new substrate for furin, Abz-Arg-Val-Lys-Arg-Gly-Leu-Ala-Tyr(NO2)-Asp-OH, has been synthesized and characterized. The peptide is an internally quenched fluorogenic substrate. The kinetic parameters are K-m = 3.8 mu M, k(cat) = 29.3 s(-1), and k(cat)/K-M = 7,710,000 M(-1) s(-1). The substrate is efficiently cleaved by furin; its k(cat)/K-M value is over 2000-fold higher than that of the commonly used substrate Boc-Arg-Val-Arg-Arg-AMC. (C) 1995 Academic Press, Inc.