MOSQUITO ECDYSTEROID RECEPTOR - ANALYSIS OF THE CDNA AND EXPRESSION DURING VITELLOGENESIS
MOSQUITO ECDYSTEROID RECEPTOR - ANALYSIS OF THE CDNA AND EXPRESSION DURING VITELLOGENESIS
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DOI：
10.1016/0965-1748(94)00045-j
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发表时间：
1995-01-01
影响因子：
3.8
通讯作者：
RAIKHEL, AS
中科院分区：
文献类型：
--
作者：
CHO, WL;KAPITSKAYA, MZ;RAIKHEL, AS
20-羟基蜕皮酮(20E)是一种昆虫类固醇激素，在蚊子卵成熟过程中起着重要的调节作用。为了更好地了解它的作用，我们克隆了埃及伊蚊(Aedes Aegypti，AaEcR)蜕皮类固醇受体的编码基因。AaEcR基因全长4158bp，开放阅读框含675个氨基酸，含有10个潜在的糖基化位点和一个可能的磷酸化多丝氨酸结构域。AaEcR具有一个带有两个锌指的DNA结合域和一个类固醇激素受体超家族成员特有的配体结合域。这些AaEcR结构域分别与果蝇蜕皮激素受体(DmEcR)的同源性分别为97%和87%。然而，AaEcR的A/B区与DmEcR-B1亚型有35%的同源性。F区位于AaEcR的羧基末端，与DmEcR的相应区域仅有9%的同源性。潜在的核靶向和二聚化信号也存在于AaEcR序列中。成虫体内有3个AaEcR转录本，分别为4.2kb、6kb和11kb。在卵黄发生过程中，4.2kb的mRNA主要在雌性蚊体中表达。在雌蚊的脂肪体和卵巢中，AaEcR基因在卵黄发生前期和卵黄发生开始后(血餐后6h)的表达水平较高。
An insect steroid hormone, 20-hydroxyecdysone (20E), plays an important role in regulating egg maturation in mosquitoes. To better understand its role, we cloned the cDNA coding for the putative ecdysteroid receptor from the mosquito, Aedes aegypti (AaEcR). The 4158 bp AaEcR cDNA has an open reading frame of 675 amino acids with 10 potential glycosylation sites and a putative phosphorylation polyserine domain. The AaEcR has a DNA binding domain with two zinc fingers and a ligand binding domain characteristic of members of the steroid hormone receptor superfamily. These AaEcR domains share 97 and 87% identities with the respective domains of the Drosophila ecdysteroid receptor (DmEcR). However, the A/B region of the AaEcR shares 35% identity with that of DmEcR-B1 isoform. The F region, located at the carboxyl-terminal of the AaEcR, has only 9% identity with the corresponding region of DmEcR. Potential nuclear targeting and dimerization signals are also present in the AaEcR sequence. There are three AaEcR transcripts of 4.2 kb, 6 kb and 11 kb in adult mosquitoes. 4.2 kb mRNA is predominantly expressed in female mosquitoes during vitellogenesis. In both the fat body and ovaries of the female mosquito, the level of AaEcR mRNA is high at the previtellogenic period and after the onset of vitellogenesis (6 h post blood meal, PBM).