Composition of human colonic mucin. Selective alteration in inflammatory bowel disease.
Composition of human colonic mucin. Selective alteration in inflammatory bowel disease.
复制标题
人结肠粘蛋白的组成。
DOI：
10.1172/jci110952
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
K. J. Isselbacher
中科院分区：
文献类型：
--
作者：
Daniel K. Podolsky;K. J. Isselbacher
人类结肠粘蛋白已从正常对照的新鲜手术标本以及克罗恩结肠炎和溃疡性结肠炎患者的粘膜刮擦中分离出来。超声和超离心后，用Sepharose 4B层析将粘蛋白与其他可溶性结肠糖蛋白分离。核酸酶消化后，用氯化铯梯度离心得到平均浮力密度为1.52 g/ml的结肠粘蛋白。随后在deae -纤维素上对明显均匀的结肠粘蛋白进行色谱分析，发现存在至少六种不同的粘蛋白（粘蛋白I-VI）。每个粘蛋白种类都有独特的己糖、己糖胺、唾液酸和硫酸盐含量以及血型物质活性。从5例克罗恩结肠炎患者的黏液蛋白中发现，与从正常结肠标本中分离出来的黏液蛋白相比，至少有6种不同的黏液蛋白。然而，在8例溃疡性结肠炎患者的黏蛋白中，有一种黏蛋白亚类成分（称为IV种）明显选择性减少。克罗恩病患者的正常黏蛋白和黏蛋白含有48 +/- 17和42 +/- 12 mg的IV种/g，而溃疡性结肠炎患者的黏蛋白含有5 +/- 3 mg/g的可溶性糖蛋白。在乙状结肠（n = 7）和升结肠（n = 4）的制剂中都发现了IV种的选择性缺失，这不能用总粘蛋白含量的总体减少来解释。在溃疡性结肠炎患者相对未发炎的结肠粘膜中分离的黏蛋白中也发现了IV种的选择性减少。其余5种粘蛋白的碳水化合物组成和血型活性与正常粘蛋白相似。根据迄今为止的结果，溃疡性结肠炎中似乎存在一种结肠粘蛋白亚类的潜在选择性减少。
Human colonic mucin has been isolated from mucosal scrapings of fresh surgical specimens of normal controls as well as patients with Crohn's colitis and ulcerative colitis. Following sonication and ultracentrifugation, mucin fractions were separated from other soluble colonic glycoproteins by Sepharose 4B chromatography. After nuclease digestion, cesium chloride gradient centrifugation of the excluded material yielded colonic mucin with an average buoyant density of 1.52 g/ml. Subsequent chromatography of the apparently homogeneous colonic mucin on DEAE-cellulose revealed the presence of at least six distinct mucin species (mucin I-VI). Each mucin species was found to have a distinctive hexose, hexosamine, sialic acid, and sulfate content as well as blood group substance activities. Mucin from five patients with Crohn's colitis was found to represent a mixture of at least six discrete species comparable to those isolated from normal colonic specimens. However, in mucin from eight patients with ulcerative colitis there was a marked and selective reduction of one component mucin subclass, designated species IV. Normal mucin and mucin from patients with Crohn's disease contained 48 +/- 17 and 42 +/- 12 mg of species IV/g, while mucin from patients with ulcerative colitis had 5 +/- 3 mg/g solubilized glycoprotein. The selective absence of species IV was found in preparations from both sigmoid (n = 7) and ascending (n = 4) colon and could not be accounted for by an overall decrease in total mucin content. The selective reduction of species IV was also found in mucin isolated from relatively noninflamed colonic mucosa of patients with ulcerative colitis. The carbohydrate composition and blood group activities of the remaining five mucin species were similar to their normal counterparts. Based on the results to date, there appears to be an underlying selective decrease of one colonic mucin subclass in ulcerative colitis.
结肠上皮粘蛋白的纯化和组成。
DOI：
10.1016/0005-2795(80)90214-7
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
LaMont,JT;Ventola,AS
通讯作者：
Ventola,AS
从大鼠结肠粘液糖蛋白中分离和表征寡糖。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Slomiany,BL;Murty,VL;Slomiany,A
通讯作者：
Slomiany,A