Gene expression changes in male accessory glands during ageing are accompanied by reproductive decline in Drosophila melanogaster
Gene expression changes in male accessory glands during ageing are accompanied by reproductive decline in Drosophila melanogaster
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DOI：
10.1111/mec.14384
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发表时间：
2017-12-01
影响因子：
4.9
通讯作者：
Fricke, Claudia
中科院分区：
文献类型：
--
作者：
Koppik, Mareike;Fricke, Claudia
衰老伴随着生殖功能的丧失。在这里，我们研究了雄性黑腹果蝇的生殖衰老，并询问雄性生殖成功率的预期下降是否由于雄性副腺（AG）功能的减弱。雄性腺产生的大部分精液蛋白（SFPs）在交配时转移给雌性腺。SFPs诱导雌性交配后的变化，是雄性繁殖成功的关键。我们测量了在羽化后1 - 6周的4个不同年龄组的雄性中5个具有代表性的SFP基因的年龄依赖性基因表达变化。同时，我们还测量了这五种SFPs转移介导的雄性交配后性状的繁殖成功率。我们发现，随着年龄的增长，雄性SFP基因的表达减少，雄性交配后的成功率也随之下降。因此，男性生殖衰老与男性AG功能下降有关。虽然单个SFP基因的表达总体上有所减少，但我们的研究结果表明，射精的成分可能随着男性年龄的增长而变化，因为这五个基因的变化率是可变的。
Senescence is accompanied by loss of reproductive functions. Here, we studied reproductive ageing in Drosophila melanogaster males and asked whether the expected decline in male reproductive success is due to diminished functionality of the male accessory gland (AG). The male AG produces the majority of seminal fluid proteins (SFPs) transferred to the female at mating. SFPs induce female postmating changes and are key to male reproductive success. We measured age-dependent gene expression changes for five representative SFP genes in males from four different age groups ranging from 1 to 6weeks after eclosion. Simultaneously, we also measured male reproductive success in postmating traits mediated by transfer of these five SFPs. We found a decreased in male SFP gene expression with advancing age and an accompanying decline in male postmating success. Hence, male reproductive senescence is associated with a decline in functionality of the male AG. While overall individual SFP genes decreased in expression, our results point towards the idea that the composition of an ejaculate might change with male age as the rate of change was variable for those five genes.