Bronchial microbiome, PA biofilm-forming capacity and exacerbation in severe COPD patients colonized by P-aeruginosa
Bronchial microbiome, PA biofilm-forming capacity and exacerbation in severe COPD patients colonized by P-aeruginosa
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DOI：
10.2217/fmb-2016-0127
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发表时间：
2017-04-01
影响因子：
3.1
通讯作者：
Monso, Eduard
中科院分区：
文献类型：
--
作者：
Garcia-Nunez, Marian;Marti, Sara;Monso, Eduard
目的：使用16 S rRNA基因测序分析了铜绿假单胞菌定植的严重慢性阻塞性肺疾病患者的支气管微生物组，以确定与生物膜形成能力相关的差异。患者与方法：对21例患者的患者痰标本进行了研究。结果如下：只有相对丰度<1%的属的生物膜形成能力存在统计学显著差异，当生物膜形成能力高时，梭杆菌属占优势。在10/14次急性加重中观察到相对丰度> 50%的属，其从基线增加，但仅在3次发作中对应于假单胞菌属，而在7次发作中鉴定出其他致病性属。结论：铜绿假单胞菌支气管微生物组的差异与其生物被膜形成能力一致。铜绿假单胞菌以外的病原微生物导致慢性阻塞性肺疾病患者的大部分急性加重。
Aim: The bronchial microbiome of severe chronic obstructive pulmonary disease patients colonized by Pseudomonas aeruginosa was analyzed using 16S rRNA gene sequencing to identify differences related to biofilm-forming capacity. Patients & methods: Patient sputum samples from 21 patients were studied. Results: Statistically significant differences related to biofilm-forming capacity were only found for genera with relative abundances < 1%, and Fusobacterium was over-represented when biofilm-forming capacity was high. Genera with relative abundances > 50% which increased from baseline were observed in 10/14 exacerbations, but corresponded to Pseudomonas only in three episodes, while other pathogenic genera were identified in seven. Conclusion: The bronchial microbiome shows differences according with P. aeruginosa biofilm-forming capacity. Pathogenic microorganisms other than P. aeruginosa cause a significant part of the exacerbations in colonized chronic obstructive pulmonary disease patients.