The HAP2,3,4 transcriptional activator is required for derepression of the yeast citrate synthase gene, CIT1.
The HAP2,3,4 transcriptional activator is required for derepression of the yeast citrate synthase gene, CIT1.
复制标题
HAP2,3,4 转录激活因子是酵母柠檬酸合酶基因 CIT1 去抑制所必需的。
DOI：
10.1111/j.1365-2958.1994.tb00407.x
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发表时间：
1994
影响因子：
3.6
通讯作者：
Devenish,LJ
中科院分区：
文献类型：
--
作者：
Rosenkrantz,M;Kell,CS;Pennell,EA;Devenish,LJ
酵母核基因CIT1编码线粒体柠檬酸合成酶，催化三羧酸(TCA)循环的第一步也是限速步骤。CIT1的转录受葡萄糖抑制。HAP2、HAP3或HAP4突变阻断了ACIT1-lacZ基因融合的去抑制。HAP2，3，4转录激活子还激活编码线粒体电子传递链组件的核基因，从而协调线粒体两个主要功能的去抑制。两个与HAP2，3，4-结合位点(ACCAATNA)相似的DNA序列位于CIT1编码序列上游约-310和-290处。缺失和突变分析表明，290元件是HAP2，3，4激活的关键。葡萄糖抑制的CIT1表达在很大程度上独立于HAP2，3，4，受谷氨酸的抑制，需要一个介于-367和-348之间的DNA序列。证据表明，第二个HAP2，3，4独立的激活元件正好位于上游，并与-290HAP2，3，4元件重叠。
The yeast nuclear geneCIT1encodes mitochondrial citrate synthase, which catalyses the first and rate‐limiting step of the tricarboxylic acid (TCA) cycle. Transcription ofCIT1is subject to glucose repression. Mutations inHAP2, HAP3orHAP4block derepression of aCIT1‐lacZgene fusion. The HAP2,3,4 transcriptional activator also activates nuclear genes encoding components of the mitochondrial electron transport chain, and thus it co‐ordinates derepression of two major mitochondrial functions. Two DNA sequences resembling the consensus HAP2,3,4‐binding site (ACCAATNA) are located at approximately‐ 310 and ‐290, upstream of theCIT1coding sequence. Deletion and mutation analysis indicates that the 290 element is critical for activation by HAP2,3,4. Glucose‐repressed expression ofCIT1is largely independent of HAP2,3,4, is repressed by glutamate, and requires a DNA sequence between ‐367 and ‐348. Evidence is presented for a second HAP2,3,4‐independent activation element located just upstream and overlapping the ‐290 HAP2,3,4 element.