CXCR5+ CCR7- CD8 T cells are early effector memory cells that infiltrate tonsil B cell follicles
CXCR5+ CCR7- CD8 T cells are early effector memory cells that infiltrate tonsil B cell follicles
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DOI：
10.1002/eji.200636746
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发表时间：
2007-12-01
影响因子：
5.4
通讯作者：
Sandberg, Johan K.
中科院分区：
文献类型：
--
作者：
Quigley, Maire F.;Gonzalez, Veronica D.;Sandberg, Johan K.
幼稚和中枢记忆CD 8 T细胞使用CCR 7再循环通过次级淋巴器官的T细胞区，在那里它们可以遇到抗原。在这里，我们描述了一个子集的人CD 8 T细胞表达CXCR 5，使归巢响应于B细胞滤泡内产生的CXCL 13。在扁桃体B细胞滤泡中发现CXCR 5（+）CD 8 T细胞，并且分离的细胞在体外向CXCL 13迁移。它们表达CD 27、CD 28、CD 45 RO、CD 69，并且是CD 7（低），并且产生IFN-γ和颗粒酶A，但缺乏穿孔素，这一功能特征表明这些细胞在当代T细胞分化模型的背景下是早期效应记忆细胞。在与B细胞的相互作用中重要的受体，包括CD 70、OX 40和ICOS，在活化后被诱导，并且CXCR 5（+）CD 8 T细胞可以在一定程度上支持扁桃体B细胞中的存活和IgG产生。此外，CXCR 5（+）CD 8 T细胞表达CCR 5，但不表达CCR 7，表明其迁移模式与滤泡CD 4 T细胞不同。早期效应记忆CD 8 T细胞亚群使用CXCR 5定位于B细胞滤泡的发现表明MHC I类限制性CD 8 T细胞是滤泡T细胞群的一部分。
Naive and central memory CD8 T cells use CCR7 to recirculate through T cell zones of secondary lymphoid organs where they can encounter antigen. Here we describe a subset of human CD8 T cells expressing CXCR5 which enables homing in response to CXCL13 produced within B cell follicles. CXCR5(+) CD8 T cells were found in tonsil B cell follicles, and isolated cells migrated towards CXCL13 in vitro. They expressed CD27, CD28, CD45RO, CD69, and were CD7(low) and produced IFN-gamma and granzyme A but lacked perforin, a functional profile suggesting that these cells are early effector memory cells in the context of contemporary T cell differentiation models. Receptors important in the interaction with B cells, including CD70, OX40 and ICOS, were induced upon activation, and CXCR5(+) CD8 T cells could to some extent support survival and IgG production in tonsil B cells. Furthermore, CXCR5(+) CD8 T cells expressed CCR5 but no CCR7, suggesting a migration pattern distinct from that of follicular CD4 T cells. The finding that a subset of early effector memory CD8 T cells use CXCR5 to locate to B cell follicles indicates that MHC class I-restricted CD8 T cells are part of the follicular T cell population.