Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina
Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina
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DOI：
10.1016/s1043-4666(02)00489-1
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发表时间：
2003-01-07
期刊：
影响因子：
3.8
通讯作者：
Aschner, JL
中科院分区：
文献类型：
--
作者：
Hassan, MI;Aschner, Y;Aschner, JL
背景：疾病的遗传易感性可能受到常见的单核苷酸多态性（SNP）形式的DNA变异的影响。SNP在连锁和关联作图研究中的价值可能取决于SNP等位基因频率在population.Objectives中的分布：在高加索和非洲裔美国妇女中建立肿瘤坏死因子（TNF）α、转化生长因子（TGF）β 1、白细胞介素-10（IL-10）、白细胞介素-6（IL-6）和干扰素（IFN）γ的SNP等位基因频率。从123名健康孕妇的全血中提取DNA。对编码TNF α（-308 G/A）、TGF β 1（密码子10 C/T、密码子25 C/G）、IL-10（-1082 A/G、-819 T/C、-592 A/C）、IL 6（-174 C/G）和IFN γ（874 T/A）的基因进行基于PCR的基因分型。等位基因频率采用Hardy-Weinberg平衡和连锁不平衡检验。高加索人和非洲裔美国人之间SNP等位基因频率的差异通过Amitage趋势检验的chi（2）进行评估。结果：白细胞介素6和干扰素γ的SNP等位基因和基因型频率在高加索人和非洲裔美国人妇女之间存在显著差异，但TNF α、TGF β 1和IL-10的SNP等位基因和基因型频率没有显著差异。识别所选细胞因子的SNP等位基因和基因型频率的种族差异对于设计和推动未来的连锁和关联作图研究是重要的，这些研究调查了细胞因子在细胞因子相关疾病中的作用。人类疾病。(C)2003爱思唯尔科技有限公司版权所有。
Background: Genetic susceptibility to diseases is likely influenced by common DNA variants in the form of single nucleotide polymorphisms (SNPs). The value of SNPs for linkage and association mapping studies may depend on the distribution of SNP allele frequencies across populations.Objectives: To establish the SNP allelic frequencies among Caucasian and African American women for tumor necrosis factor (TNF)alpha, transforming growth factor (TGF)beta1, interleukin-10 (IL10), interleukin-6 (IL6), and interferon (IFN)gamma.Materials and methods: DNA was extracted from whole blood from 123 healthy, pregnant women. PCR-based genotyping was performed for the genes encoding TNFalpha (-308G/A), TGFbeta1 (codon 10C/T, codon 25C/G), IL-10 (-1082A/G, -819T/C, -592A/C), IL6 (-174C/G) and IFNgamma (874T/A). Allele frequencies were determined by Hardy-Weinberg Equilibrium and Linkage Disequilibrium tests. Differences in the SNP allelic frequencies between Caucasians and African Americans were assessed by the chi(2) of Amitage trend test.Results: SNP allelic and genotypic frequencies for IL6 and IFNgamma, but not for TNFalpha, TGFbeta1, and IL-10, differed significantly between the Caucasian and African American women.Conclusions: Recognition of racial differences in SNP allelic and genotypic frequencies for selected cytokines is important for designing and powering future linkage and association mapping studies investigating the role of cytokines in human disease. (C) 2003 Elsevier Science Ltd. All rights reserved.